Bode H B, Zeeck A
Institut für Organische Chemie, Universität Göttingen, Germany.
Phytochemistry. 2000 Oct;55(4):311-6. doi: 10.1016/s0031-9422(00)00307-1.
The metabolite pattern of UV mutants of the spirobisnaphthalene producing fungus F-24'707 by TLC and HPLC analysis has been investigated. Mutants with differences in colony morphology or colour compared to the parent strain were isolated. Cultivation in shaking flasks and P flasks showed differences in the metabolite pattern of some of the strains. Furthermore, enzyme inhibitors were used to block the spirobisnaphthalene biosynthesis of the parent strain at different steps. Feeding of precursors and intermediates of cladospirone bisepoxide (15) led to a two-fold increase of the production of 15. From these data and preceding biosynthetic studies we deduced a general pathway for the biosynthesis of all spirobisnaphthalenes of the fungus F-24'707. This enables us to present the hypothesis that all bisnaphthalenes described so far are produced using a common pathway with only a few intermediates as central branching points.
通过薄层层析（TLC）和高效液相色谱（HPLC）分析，对产生螺二萘的真菌F-24'707的紫外线突变体的代谢物模式进行了研究。分离出了与亲本菌株相比菌落形态或颜色不同的突变体。在摇瓶和P瓶中培养显示，一些菌株的代谢物模式存在差异。此外，使用酶抑制剂在不同步骤阻断亲本菌株的螺二萘生物合成。添加双环氧枝顶孢菌素（15）的前体和中间体导致15的产量增加了两倍。根据这些数据和之前的生物合成研究，我们推导了真菌F-24'707所有螺二萘生物合成的一般途径。这使我们能够提出一个假设，即到目前为止描述的所有双萘都是使用一条共同途径产生的，只有少数中间体作为中心分支点。
