Takano N, Nishikura K
Experientia. 1976 Nov 15;32(11):1437-9. doi: 10.1007/BF01937419.
The Haldane coefficient (the amount of the oxygen-linked hydrogen ion binding of hemoglobin) was determined in bovine erythrolysate (Hb concentration equals 13.5 mM) by means of the differential titration method with varying PCO2 from 0 to 74 mm Hg and pH from 6.0 to 8.5 at 37 degrees C. The maximum value of the coefficient was found to be 0.49 mM per mM Hb at PCO2 equals 0 and pH 7.20. With increasing of PCO2, the coefficient became smaller in all ranges of pH studied. The coefficient under the conditions of pH 7.20 and PCO2 equals 45 mm Hg that are normally prevailing in the interior of bovine erythrocytes was 0.31.
通过微分滴定法，在37摄氏度下，于不同二氧化碳分压（从0至74毫米汞柱）和pH值（从6.0至8.5）条件下，测定了牛红细胞溶解产物（血红蛋白浓度等于13.5毫摩尔）中的哈代系数（血红蛋白与氧结合的氢离子结合量）。发现该系数在二氧化碳分压为0且pH值为7.20时的最大值为每毫摩尔血红蛋白0.49毫摩尔。随着二氧化碳分压的升高，在所研究的所有pH范围内，该系数均变小。在牛红细胞内部通常存在的pH值为7.20且二氧化碳分压等于45毫米汞柱的条件下，该系数为0.31。
