用于人类钩端螺旋体病快速血清学诊断的侧向流动分析法。
Lateral-flow assay for rapid serodiagnosis of human leptospirosis.
作者信息
Smits H L, Eapen C K, Sugathan S, Kuriakose M, Gasem M H, Yersin C, Sasaki D, Pujianto B, Vestering M, Abdoel T H, Gussenhoven G C
机构信息
Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
出版信息
Clin Diagn Lab Immunol. 2001 Jan;8(1):166-9. doi: 10.1128/CDLI.8.1.166-169.2001.
Abstract
An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.
摘要
本文介绍了一种用于快速检测人血清中钩端螺旋体特异性免疫球蛋白M（IgM）抗体的检测装置。该检测方法的灵敏度（85.8%）和特异性（93.6%）与通常用于钩端螺旋体病血清诊断的IgM酶联免疫吸附测定法相当（一致性为91.9%）。该检测方法的灵敏度随疾病阶段而变化。该检测使用稳定的成分，只需将血清和样本液加入检测装置的样本孔中即可简单操作。10分钟后读取检测结果，当观察到检测线染色时即为阳性结果。
相似文献
1
Lateral-flow assay for rapid serodiagnosis of human leptospirosis.
Clin Diagn Lab Immunol. 2001 Jan;8(1):166-9. doi: 10.1128/CDLI.8.1.166-169.2001.
2
Introduction of a rapid dipstick assay for the detection of Leptospira-specific immunoglobulin m antibodies in the laboratory diagnosis of leptospirosis in a hospital in Makassar, Indonesia.
Southeast Asian J Trop Med Public Health. 2000 Sep;31(3):515-20.
3
Western immunoblot analysis using a ten leptospira serovars combined antigen for serodiagnosis of leptospirosis.
Southeast Asian J Trop Med Public Health. 2006 Mar;37(2):309-11.
4
Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis.
Comp Immunol Microbiol Infect Dis. 2010 Sep;33(5):375-87. doi: 10.1016/j.cimid.2009.01.001. Epub 2009 Mar 23.
5
Evaluation of the clinical utility of a rapid blood test for human leptospirosis.
Diagn Microbiol Infect Dis. 2002 Apr;42(4):221-5. doi: 10.1016/s0732-8893(01)00361-3.
6
A test strip IgM dot-ELISA assay using leptospiral antigen of endemic strains for serodiagnosis of acute leptospirosis.
J Med Assoc Thai. 2005 Mar;88(3):391-8.
7
Two simple immunoassays using endemic leptospiral antigens for serodiagnosis of human leptospirosis.
Southeast Asian J Trop Med Public Health. 2005 Mar;36(2):302-11.
8
Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis.
Indian J Med Microbiol. 2010 Jul-Sep;28(3):238-40. doi: 10.4103/0255-0857.66484.
9
Comparison of a slide agglutination test, LeptoTek Dri-Dot, and IgM-ELISA with microscopic agglutination test for Leptospira antibody detection.
Southeast Asian J Trop Med Public Health. 2007 Nov;38(6):1111-4.
10
Recombinant ligA for leptospirosis diagnosis and ligA among the Leptospira spp. clinical isolates.
J Microbiol Methods. 2008 Jan;72(1):73-81. doi: 10.1016/j.mimet.2007.10.012. Epub 2007 Nov 17.
引用本文的文献
1
Production of recombinant electron transfer flavoprotein beta subunit protein and its application in a lateral flow assay for early diagnosis of leptospirosis.
Med Microbiol Immunol. 2025 Feb 19;214(1):12. doi: 10.1007/s00430-025-00822-6.
2
Accuracy of rapid lateral flow immunoassays for human leptospirosis diagnosis: A systematic review and meta-analysis.
PLoS Negl Trop Dis. 2024 May 15;18(5):e0012174. doi: 10.1371/journal.pntd.0012174. eCollection 2024 May.
3
Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography.
Mem Inst Oswaldo Cruz. 2023 May 12;118:e220265. doi: 10.1590/0074-02760220265. eCollection 2023.
4
Evaluating immunochromatographic test kits for diagnosis of acute human leptospirosis: A systematic review.
Heliyon. 2022 Nov 24;8(11):e11829. doi: 10.1016/j.heliyon.2022.e11829. eCollection 2022 Nov.
5
A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.
Curr Issues Mol Biol. 2022 Sep 21;44(10):4367-4385. doi: 10.3390/cimb44100300.
6
Lateral flow assay for rapid serodiagnosis of bovine leptospirosis.
Iran J Vet Res. 2022;23(1):7-11. doi: 10.22099/IJVR.2021.41151.5974.
7
Enzyme immunoassays (EIA) for serodiagnosis of human leptospirosis: specific IgG3/IgG1 isotyping may further inform diagnosis of acute disease.
PLoS Negl Trop Dis. 2022 Feb 23;16(2):e0010241. doi: 10.1371/journal.pntd.0010241. eCollection 2022 Feb.
8
Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection.
J Nanobiotechnology. 2022 Jan 4;20(1):6. doi: 10.1186/s12951-021-01188-6.
9
Comparative Study of Indirect Fluorescent Antibody, ELISA, and Immunochromatography Tests for Serological Diagnosis of Bovine Babesiosis Caused by .
Animals (Basel). 2021 Nov 24;11(12):3358. doi: 10.3390/ani11123358.
10
Regional Prevalence of Intermediate spp. in Humans: A Meta-Analysis.
Pathogens. 2021 Jul 27;10(8):943. doi: 10.3390/pathogens10080943.
本文引用的文献
1
A rapid macroscopicslide screening test for the serodiagnosis of leptospirosis.
Am J Vet Res. 1958 Apr;19(71):505-12.
2
International multi-centre evaluation of a dipstick assay for human leptospirosis.
Trop Med Int Health. 2000 Feb;5(2):124-8. doi: 10.1046/j.1365-3156.2000.00525.x.
3
Increase of leptospirosis in dengue-negative patients after a hurricane in Puerto Rico in 1996 [correction of 1966].
Am J Trop Med Hyg. 1999 Sep;61(3):399-404. doi: 10.4269/ajtmh.1999.61.399.
4
Urban epidemic of severe leptospirosis in Brazil. Salvador Leptospirosis Study Group.
Lancet. 1999 Sep 4;354(9181):820-5. doi: 10.1016/s0140-6736(99)80012-9.
5
Leptospiral infection among primitive tribes of Andaman and Nicobar Islands.
Epidemiol Infect. 1999 Jun;122(3):423-8. doi: 10.1017/s0950268899002435.
6
LEPTO Dipstick: a rapid and simple method for serodiagnosis of acute leptospirosis.
Trans R Soc Trop Med Hyg. 1999 Mar-Apr;93(2):161-4. doi: 10.1016/s0035-9203(99)90293-6.
7
International multicenter evaluation of the clinical utility of a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human serum specimens.
J Clin Microbiol. 1999 Sep;37(9):2904-9. doi: 10.1128/JCM.37.9.2904-2909.1999.
8
Leptospirosis worldwide, 1999.
Wkly Epidemiol Rec. 1999 Jul 23;74(29):237-42.
9
Leptospirosis in Philippine floods.
BMJ. 1999 Jul 24;319(7204):212.
10
Field evaluation of a one-step dipstick assay for the diagnosis of human leptospirosis in the Seychelles.
Trop Med Int Health. 1999 Jan;4(1):38-45. doi: 10.1046/j.1365-3156.1999.00352.x.
Zotero 插件