De Paepe K, Lagarde J M, Gall Y, Roseeuw D, Rogiers V
Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Arch Dermatol Res. 2000 Oct;292(10):500-10. doi: 10.1007/s004030000166.
The recently developed Skin Visiometer, based on light transmission through blue-coloured silicone replicas, was used to study skin microrelief. Calibrated metal plates displaying lines with depths between 6 and 361 microns, were used to determine the accuracy, sensitivity and reproducibility of the technique as well as the parameters of importance during measurement. The precision of the measurements was particularly good between 10 microns and 361 microns. The sensitivity of the method was between 10 and 20 microns. Replicas of volar forearm skin were taken from four groups (n = 15) of male and female volunteers in the age ranges 20 to 30 years and 55 to 65 years. In addition to the instrumental roughness parameters (Rz, Rt, Rm and Ra), the surface of the furrows, the number of primary and secondary lines and the number of intersections were determined. For both sexes, significantly lower values were observed for Rz, Rm and Rt in the younger age group than in the older age group. In addition, the numbers of primary and secondary lines and the number of intersections were higher, pointing to a more structured microrelief in younger forearm skin. Diurnal rhythm, the relative humidity of the measuring room and the position of the forearm were found to be significant factors, while room temperature and precleansing of the skin with mild products were not. Following the application of a hydrating cream (twice daily for 14 days) to the forearm skin of the older female age group, the Rz, Rt, Rm and Ra decreased, while the other parameters measured, except for the surface taken in by the lines, increased, indicating that the microrelief was modified towards the typical pattern observed in young skin.
最近研发的皮肤表面光洁度仪，基于光线透过蓝色硅胶复制品的原理，用于研究皮肤微观起伏。使用校准后的金属板，其上刻有深度在6至361微米之间的线条，以确定该技术的准确性、灵敏度和可重复性，以及测量过程中的重要参数。测量精度在10微米至361微米之间特别好。该方法的灵敏度在10至20微米之间。从年龄在20至30岁和55至65岁的四组（每组n = 15）男性和女性志愿者的掌侧前臂皮肤上获取复制品。除了仪器粗糙度参数（Rz、Rt、Rm和Ra）外，还测定了皱纹表面、主次线条数量和交叉点数量。对于两性而言，较年轻年龄组的Rz、Rm和Rt值明显低于较年长年龄组。此外，主次线条数量和交叉点数量更多，表明年轻前臂皮肤的微观起伏结构更复杂。发现昼夜节律、测量室的相对湿度和前臂位置是重要因素，而室温以及用温和产品对皮肤进行预清洁则不是。在老年女性年龄组的前臂皮肤上每天涂抹两次保湿霜，持续14天之后，Rz、Rt、Rm和Ra降低，而所测量的其他参数（除线条所占据的表面外）增加，这表明微观起伏朝着在年轻皮肤中观察到的典型模式发生了改变。
