Jones L, Tucci M, Benghuzzi H
School of Health Related Professions, University of Mississippi Medical Center, Jackson 39216, USA.
Biomed Sci Instrum. 1997;34:53-8.
The purpose of this experiment was to determine the effect of TCP, ALCAP, and HA ceramic particles on the viability and proliferation rate of HL-60 cells in culture. Previous experiments have suggested that growing cell lines such as macrophages, fibroblasts, and endothelial cells on discs made of TCP, ALCAP, and HA induced immunochemical and morphological changes. The effect of ceramic particles (< 38 um) on HL-60 cells in culture have never been investigated. A total of 1 x 10(5) (n = 5) cells were placed in culture with 2 mg of ALCAP, TCP, HA particles, or 10 micrograms/ml lipopolysaccharides (LPS). At the end of 24, 48, and 96 hours, the cells were evaluated morphologically and then counted. The supernatants were collected and biochemical analysis for LDH and immunochemical analysis for cytokines IL-1 was performed. The data from this investigation revealed the following: (1) At the end of the 24 hour phase, the cell number in wells containing HA and ALCAP particles were lower than the control cells, whereas cells in the presence of TCP were similar to the control. (2) Microscopic evaluation of cells treated with HA showed hydropic change at 24, 48 and 96 hours. (3) At 96 hours, there were no significant changes in cell number between the groups. This phenomenon could be attributed to the depletion of nutrients within the wells or possibly the cells reaching confluence. (4) The ease of ingestion of macrophages to CPB is as follows: TCP > ALCAP > HA. (5) Biochemical analysis of markers for cellular disturbances revealed that there were no significant differences between the different group at any time point (p < 0.05).
本实验的目的是确定磷酸三钙（TCP）、碱性磷酸钙（ALCAP）和羟基磷灰石（HA）陶瓷颗粒对培养的HL-60细胞活力和增殖率的影响。先前的实验表明，在由TCP、ALCAP和HA制成的圆盘上培养巨噬细胞、成纤维细胞和内皮细胞等细胞系会诱导免疫化学和形态学变化。陶瓷颗粒（<38微米）对培养的HL-60细胞的影响从未被研究过。将总共1×10⁵个细胞（n = 5）与2毫克的ALCAP、TCP、HA颗粒或10微克/毫升脂多糖（LPS）一起培养。在24、48和96小时结束时，对细胞进行形态学评估，然后计数。收集上清液，进行乳酸脱氢酶（LDH）的生化分析和细胞因子白细胞介素-1（IL-1）的免疫化学分析。本次研究的数据显示如下：（1）在24小时阶段结束时，含有HA和ALCAP颗粒的孔中的细胞数量低于对照细胞，而存在TCP的孔中的细胞与对照相似。（2）用HA处理的细胞在24、48和96小时的显微镜评估显示出现水肿变化。（3）在96小时时，各组之间的细胞数量没有显著变化。这种现象可能归因于孔内营养物质的耗尽或可能是细胞达到汇合状态。（4）巨噬细胞对CPB的摄取难易程度如下：TCP>ALCAP>HA。（5）细胞紊乱标志物的生化分析表明，在任何时间点不同组之间均无显著差异（p<0.05）。
