三名胭脂红过敏患者中胭脂红过敏原的鉴定。

Identification of carmine allergens among three carmine allergy patients.

作者信息

Chung K, Baker J R, Baldwin J L, Chou A

机构信息

Department of Internal Medicine, University of Michigan Hospital, Ann Arbor 48109-0380, USA.

出版信息

Allergy. 2001 Jan;56(1):73-7. doi: 10.1034/j.1398-9995.2001.00693.x.

DOI:10.1034/j.1398-9995.2001.00693.x

PMID:11167356

Abstract

BACKGROUND

There have been several reports of carmine allergy; however, identification of the responsible carmine allergens has not been widely documented.

METHODS

Three female patients presented with a history of anaphylaxis and/or urticaria/angioedema after ingestion of carmine-containing foods. All three patients had 4+ skin prick tests to carmine. Among them, two patients were confirmed to have carmine allergy by blinded, placebo-controlled food challenges to carmine. SDS-PAGE of cochineal insects and carmine, immunoblotting for IgE antibody with sera from all three patients, and immunoblotting inhibition with carmine were performed.

RESULTS

SDS PAGE of minced cochineal insects revealed several protein bands of 23-88 kDa. Several of these bands were variably recognized by our three patients' sera, and this reactivity was inhibited by carmine. Although no protein bands could be visualized on SDS-PAGE of carmine in Coomassie brilliant blue staining, three protein bands were recognized by two of the three patients' serum.

CONCLUSIONS

These results suggest that commercial carmine retains protein-aceous material from the source insects. These insect-derived proteins (possibly complexed with carminic acid) are responsible for IgE-mediated carmine allergy. Patient reactivity to these proteins may vary.

摘要

背景

已有数篇关于胭脂红过敏的报道；然而，对相关胭脂红过敏原的鉴定尚未得到广泛记录。

方法

三名女性患者在摄入含胭脂红的食物后出现过敏反应和/或荨麻疹/血管性水肿病史。所有三名患者对胭脂红的皮肤点刺试验结果均为4+。其中两名患者通过对胭脂红进行双盲、安慰剂对照的食物激发试验，被确诊为胭脂红过敏。对胭脂虫和胭脂红进行了十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE），用三名患者的血清进行了IgE抗体免疫印迹分析，并用胭脂红进行了免疫印迹抑制试验。