通过DNA复性动力学对噬菌体Mu病毒粒子中不均匀DNA的表征。
Characterization of the inhomogeneous DNA in virions of bacteriophage Mu by DNA reannealing kinetics.
作者信息
Daniell E, Kohne D E, Abelson J
出版信息
J Virol. 1975 Apr;15(4):739-43. doi: 10.1128/JVI.15.4.739-743.1975.
Abstract
The DNA of bacteriophage Mu has been studied to characterize a region of inhomogeneous sequence that occurs at one end of the molecule. The kinetics of reassocation of tracer amounts of labeled host DNA in the presence of Mu DNA show that Mu DNA contains a complete selection of host sequences. These host sequences are shown to be covalently attached to phage-specific sequences and are present at a concentration that accounts for the inhomogeneity observed in the electron microscope. The significance and possible function of the host DNA attachment is discussed.
摘要
对噬菌体Mu的DNA进行了研究，以表征分子一端出现的不均一序列区域。在Mu DNA存在的情况下，标记宿主DNA示踪量的重缔合动力学表明，Mu DNA包含宿主序列的完整选择。这些宿主序列被证明与噬菌体特异性序列共价连接，其存在浓度解释了在电子显微镜下观察到的不均一性。讨论了宿主DNA附着的意义和可能的功能。
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