Department of Endodontology, School of Dentistry, Ege University, 35100, İzmir, Turkey.
Department of Bioengineering, Faculty of Engineering, Ege University, İzmir, Turkey.
Clin Oral Investig. 2023 Sep;27(9):5413-5425. doi: 10.1007/s00784-023-05160-6. Epub 2023 Jul 24.
The aim of this study was to investigate solubility, pH value, chemical structure, radiopacity, and cytotoxicity of AH Plus BC, TotalFill BC, AH Plus, and AH Plus Jet sealers.
Cytotoxicity analysis with direct and extraction tests at 3 different concentrations (1:1, 1:2, 1:4 v/v%) and time (24 h, 48 h, and 72 h) on Saos-2, PdLF, and THP-1 cell lines, chemical structure with scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis, solubility, pH, and radiopacity values of AH Plus BC, TotalFill BC, AH Plus, and AH Plus Jet were evaluated. For statistical analyses of the groups, repeated measures, factorial, and one-way ANOVA tests were used. The statistical significance level was set at p < .05.
Resin-based sealers showed higher cytotoxicity values than the bioceramic-based sealers (p < 0.05). Time and concentrations were effective on the cell viabilities for cell lines. Higher peaks of calcium were detected bioceramic-based sealers and higher amount of zirconium was detected in AH Plus BC (p < 0.05). AH Plus BC showed similar radiopacity value with AH Plus, AH Plus Jet, whereas TotalFill BC showed the lowest radiopacity (p < 0.05). Bioceramic-based sealers had higher pH values in all experiment periods, and the difference between resin- and bioceramic-based sealer groups was significant (p < 0.05). However, the solubility values of the tested root canal sealers revealed no differences (p > 0.05).
The newly produced AH Plus BC Sealer showed similar properties with TotalFill BC, and their biological properties were better than AH Plus and AH Plus Jet.
AH Plus BC could be a possible alternative to other bioceramic- or resin-based sealers.
本研究旨在研究 AH Plus BC、TotalFill BC、AH Plus 和 AH Plus Jet 根管封闭剂的溶解度、pH 值、化学结构、射线可透性和细胞毒性。
采用直接和提取试验，在 3 种不同浓度（1:1、1:2、1:4 v/v%）和时间（24 h、48 h 和 72 h）下，对 Saos-2、PdLF 和 THP-1 细胞系进行细胞毒性分析，采用扫描电子显微镜（SEM）和能谱分析（EDX）分析化学结构，评价 AH Plus BC、TotalFill BC、AH Plus 和 AH Plus Jet 的溶解度、pH 值和射线可透性值。对各组数据进行统计分析，采用重复测量、析因和单因素方差分析。统计显著性水平设定为 p <.05。
树脂基封闭剂的细胞毒性值高于生物陶瓷基封闭剂（p < 0.05）。时间和浓度对细胞活力有影响。生物陶瓷基封闭剂中检测到更高的钙峰，AH Plus BC 中检测到更高的锆含量（p < 0.05）。AH Plus BC 与 AH Plus、AH Plus Jet 具有相似的射线可透性值，而 TotalFill BC 则具有最低的射线可透性（p < 0.05）。在所有实验期间，生物陶瓷基封闭剂的 pH 值均较高，树脂基和生物陶瓷基封闭剂组之间的差异具有统计学意义（p < 0.05）。然而，测试的根管封闭剂的溶解度值没有差异（p > 0.05）。
新生产的 AH Plus BC 封闭剂与 TotalFill BC 具有相似的特性，其生物学性能优于 AH Plus 和 AH Plus Jet。
AH Plus BC 可能是其他生物陶瓷基或树脂基封闭剂的一种替代选择。
