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噬菌体G101(铜绿假单胞菌)区域c的基因定位

Genetic mapping of the region c of the bacteriophage G101 (Pseudomonas aeruginosa).

作者信息

Spanová A

出版信息

Folia Microbiol (Praha). 1979;24(3):217-23. doi: 10.1007/BF02926451.

Abstract

Morphological mutants of the c type of the bacteriophage G101 (Pseudomonas aeruginosa) were isolated after mutagenesis with hydroxylamine. Complementation analysis of 27 c mutants showed that the c region is formed by at least two genes. Two types of c mutants were obtained. One of them (cI26) behaves analogously to a mutant in the gene controlling the synthesis of the repressor of phage lambda. The second type of the c mutants (cII1, cII18) specifies a gene having probably an auxiliary function in the "c" region. According to the low frequency of recombination between the genes cI26 and c II18 (1.37 recombination units), these genes responsible for lysogenization are localized in a short region of the chromosome.

摘要

用羟胺诱变后,分离出了噬菌体G101(铜绿假单胞菌)c型的形态突变体。对27个c突变体的互补分析表明,c区域至少由两个基因组成。获得了两种类型的c突变体。其中一种(cI26)的行为类似于噬菌体λ阻遏物合成控制基因中的突变体。第二种类型的c突变体(cII1、cII18)指定了一个可能在“c”区域具有辅助功能的基因。根据cI26和cII18基因之间的低重组频率(1.37个重组单位),这些负责溶原化的基因位于染色体的一个短区域。

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