铜绿假单胞菌溶原性建立缺陷突变体的特性分析
Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny.
作者信息
Miller R V, Ku C M
出版信息
J Bacteriol. 1978 Jun;134(3):875-83. doi: 10.1128/jb.134.3.875-883.1978.
Abstract
Mutants of Pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (Les-) have been isolated. These les mutations map to two areas of the P. aeruginosa chromosomal map as determined by conjugational and transductional analyses. Two phenotypic classes of Les- mutants were identified. One class of mutations has pleiotropic effects on DNA metabolism. These mutants are unable to recombine genetic material acquired as a result of either conjugation or transduction (Rec-). In addition, the ability of these Les- Rec- mutants to repair UV-induced damage to bacteriophage is reduced (host-cell reactivation deficient, Hcr-). Mutants of the second class are Les-, Rec+, and Hcr+.
摘要
已分离出与温和噬菌体建立溶原关系能力受损的铜绿假单胞菌突变体（Les-）。通过接合和转导分析确定，这些les突变定位于铜绿假单胞菌染色体图谱的两个区域。鉴定出了两类Les-突变体的表型。一类突变对DNA代谢具有多效性影响。这些突变体无法重组因接合或转导而获得的遗传物质（Rec-）。此外，这些Les-Rec-突变体修复紫外线诱导的噬菌体损伤的能力降低（宿主细胞再活化缺陷，Hcr-）。第二类突变体是Les-、Rec+和Hcr+。
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