Guérin-Danan C, Meslin J C, Chambard A, Charpilienne A, Relano P, Bouley C, Cohen J, Andrieux C
INRA, UEPSD, MBS, 78352 Jouy en Josas cedex, France.
J Nutr. 2001 Jan;131(1):111-7. doi: 10.1093/jn/131.1.111.
Group A rotavirus is the leading cause of diarrhea among children aged 3-36 mo worldwide. Introducing fermented milk products into the infant diet has been proposed for the prevention or treatment of rotavirus diarrhea. The preventive effect of milk fermented by the Lactobacillus casei strain DN-114 001 was studied in a model of germfree suckling rats supplemented daily from d 2 of life and infected with SA11 rotavirus at d 5 (RF group). One group was supplemented with nonfermented milk (RM) and two uninfected groups (CM and CF) received either nonfermented or fermented milk. Frequency and severity of diarrhea were observed. Rats were killed at various times from 0 to 120 h postinfection (p.i.). Bacteria were measured in the intestine, and rotavirus antigens were detected by ELISA in fecal samples and in different parts of the intestine. Histologic observations were made, including vacuolation, morphology of intestinal villi and number of mucin cells. RM rats had diarrhea for 6 d; compared with the CM group, they had alterations of the intestinal mucosa characterized by cellular vacuolation 48 and 72 h p.i. and a lower number of sulfated mucin cells 72 and 96 h p.i. (P: < 0.05). Early supplementation with fermented milk significantly decreased the clinical signs of diarrhea from 24 to 144 h p.i. (P: < 0.05) and prevented rotavirus infection in all sections of the intestine. Histologic lesions of the small intestine were greatly reduced (P: < 0.05) and the number of mucin cells remained unchanged. The data are discussed with respect to the possibility of reducing rotavirus diarrhea in young children by consumption of fermented milk.
A组轮状病毒是全球3至36个月大儿童腹泻的主要病因。有人提议在婴儿饮食中引入发酵乳制品以预防或治疗轮状病毒腹泻。在无菌乳鼠模型中研究了干酪乳杆菌DN-114 001发酵乳的预防效果，这些乳鼠从出生第2天开始每天补充该发酵乳，并在第5天感染SA11轮状病毒（RF组）。一组补充未发酵乳（RM），两个未感染组（CM和CF）分别接受未发酵或发酵乳。观察腹泻的频率和严重程度。在感染后0至120小时的不同时间处死大鼠。检测肠道中的细菌，并通过ELISA在粪便样本和肠道不同部位检测轮状病毒抗原。进行组织学观察，包括空泡形成、肠绒毛形态和黏蛋白细胞数量。RM组大鼠腹泻6天；与CM组相比，它们在感染后48和72小时肠道黏膜出现以细胞空泡形成为特征的改变，在感染后72和96小时硫酸化黏蛋白细胞数量减少（P<0.05）。早期补充发酵乳显著降低了感染后24至144小时腹泻的临床症状（P<0.05），并预防了肠道所有部位的轮状病毒感染。小肠的组织学损伤大大减轻（P<0.05），黏蛋白细胞数量保持不变。针对通过食用发酵乳减少幼儿轮状病毒腹泻的可能性对这些数据进行了讨论。
