Bharathi S, Lev O
Fredy and Nadine Herrmann School of Applied Sciences, Hebrew University of Jerusalem, Jerusalem, 91904 Israel.
Appl Biochem Biotechnol. 2000 Nov-Dec;89(2-3):209-16. doi: 10.1385/abab:89:2-3:209.
A new type of inorganic biosensor is introduced. The sensor comprises glucose oxidase enzymes encapsulated in a sol-gel-derived Prussian blue-silicate hybrid network. Glucose is detected by the biocatalytic reduction of oxygen followed by catalytic reduction of hydrogen peroxide by the Prussian blue catalyst. The sol-gel silicate entails a rigid encapsulating matrix, the Prussian blue provides chemical catalysis and charge mediation from the reduction site to the supporting electrode, and the enzyme is responsible for the biocatalysis. The feasibility of a dual optical/electrochemical mode of analysis is also demonstrated.
介绍了一种新型无机生物传感器。该传感器由封装在溶胶-凝胶衍生的普鲁士蓝-硅酸盐混合网络中的葡萄糖氧化酶组成。通过氧气的生物催化还原，随后由普鲁士蓝催化剂催化还原过氧化氢来检测葡萄糖。溶胶-凝胶硅酸盐形成一个刚性封装基质，普鲁士蓝提供化学催化作用以及从还原位点到支撑电极的电荷传导，而酶负责生物催化作用。还证明了双光学/电化学分析模式的可行性。
