Navarro P, Giner R M, Recio M C, Máñez S, Cerdá-Nicolás M, Ríos J L
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.
Life Sci. 2001 Jan 26;68(10):1199-206. doi: 10.1016/s0024-3205(00)01019-5.
Seven oleanane-type triterpene saponins were isolated from the methanolic extract of the aerial parts of Bupleurum rotundifolium. They were identified on the basis of their spectral data as 3-O-[alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl]-28-O-[beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl] echinocystic acid (saponin 1), 3-O-[alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranosyl (1-->2)-beta-D-fucopyranosyl] 11-methoxy-primulagenin A (saponin 2), rotundioside E (saponin 3), rotundioside F (saponin 4), 3beta-sulfate, 28-O-[beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl] ester of primulagenin A (saponin 5), rotundioside C (saponin 6) and 3-O-[alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranosyl (1-->2)-beta-D-fucopyranosyl] 11-methoxy-16beta,21alpha,28-trihydroxyolean-12-ene (saponin 7). All these saponins proved to be effective against TPA-induced ear edema in mice. Their ID50 were determined to be 248, 288, 128, 99 and 297 nmol/ear for saponin 1, 2, 3, 4 and 6, respectively. Saponins 3 and 6 were also active on a TPA multiple-dose model of skin chronic inflammation.
从圆叶柴胡地上部分的甲醇提取物中分离出七种齐墩果烷型三萜皂苷。根据它们的光谱数据，将其鉴定为3-O-[α-L-鼠李吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基]-28-O-[β-D-葡萄糖吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基]刺囊酸（皂苷1）、3-O-[α-L-鼠李吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基(1→2)-β-D-岩藻糖吡喃糖基]11-甲氧基-报春花苷元A（皂苷2）、圆叶皂苷E（皂苷3）、圆叶皂苷F（皂苷4）、3β-硫酸盐、报春花苷元A的28-O-[β-D-葡萄糖吡喃糖基(1→6)-β-D-葡萄糖吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基]酯（皂苷5）、圆叶皂苷C（皂苷6）和3-O-[α-L-鼠李吡喃糖基(1→2)-β-D-葡萄糖吡喃糖基(1→2)-β-D-岩藻糖吡喃糖基]11-甲氧基-16β,21α,28-三羟基齐墩果-12-烯（皂苷7）。所有这些皂苷都被证明对小鼠TPA诱导的耳部水肿有效。皂苷1、2、3、4和6的半数抑制剂量（ID50）分别测定为248、288、128、99和297 nmol/耳。皂苷3和6在TPA多剂量皮肤慢性炎症模型中也具有活性。
