Hopkins R, Robertson M, Ross D, Turnbull W M, Das P C
Br Med J. 1975 May 24;2(5968):409-11. doi: 10.1136/bmj.2.5968.409.
A total of 70 224 blood donations were tested at three Scottish blood transfusion centres for hepatitis B surface antigen /HBsAg) by an economical haemagglutination-inhibition method (E.H.A.I.) and the results compared with those of counterelectrophoresis (C.E.P.). A further 4086 donations were tested using the Wellcome turkey cell haemagglutination test, C.E.P., and E.H.A.I.E.H.A.I. was also compared with commercial haemagglutination and radioimmunoassay reagents for sensitivity and specificity against several established antigen panels and used to reinvestigate counterelectrophoresis-negative blood donations implicated in post-transfusion hepatitis. E.H.A.I. combines the inherent specificity of an inhibition reaction with a sensitivity equal to that of commercial radioimmunoassay and haemagglutination kits but at a fraction of the cost. The assessment of 70 224 blood donations in three regions showed that E.H.A.I. detected more antigen-positive blood donations than C.E.P. Results of retesting more than 100 blood donors implicated in 10 cases of post-transfusion hepatitis suggested that the use of E.H.A.I. or a test of similar sensitivity in place of C.E.P. may significantly reduce the incidence of this complication.
在苏格兰的三个输血中心，采用经济的血凝抑制法（E.H.A.I.）对总共70224份献血进行了乙型肝炎表面抗原（HBsAg）检测，并将结果与对流电泳（C.E.P.）的结果进行比较。另外4086份献血使用了威康火鸡细胞血凝试验、C.E.P.和E.H.A.I.进行检测。E.H.A.I.还与商业血凝和放射免疫分析试剂就针对几种既定抗原组的敏感性和特异性进行了比较，并用于重新检测与输血后肝炎有关的对流电泳阴性献血。E.H.A.I.将抑制反应的固有特异性与等同于商业放射免疫分析和血凝试剂盒的敏感性相结合，但成本仅为其几分之一。对三个地区70224份献血的评估表明，E.H.A.I.检测出的抗原阳性献血比C.E.P.更多。对涉及10例输血后肝炎的100多名献血者进行重新检测的结果表明，使用E.H.A.I.或类似敏感性的检测方法替代C.E.P.可能会显著降低这种并发症的发生率。
