Tachibana C F, Baba K, Fukuda M, Imai M, Miyakawa Y, Mayumi M
Vox Sang. 1977;32(5):296-9. doi: 10.1111/j.1423-0410.1977.tb00649.x.
Hepatitis B surface antigen (HBsAg) was detected by an immune adherence haemagglutination method in the serum samples of 292 voluntary, apparently healthy blood donors at four regional blood centres in Japan. Their serum samples were concentrated 3-fold and tested for e antigen (e Ag) and antibody to e (anti-e) by immunodiffusion. The e Ag was found in 41 samples (14.0%) and anti-e in 57 (18.6%). When 100 randomly selected serum samples containing HBsAg were tested as they were (unconcentrated), and at 3- and 5-fold concentrations, e Ag was detected in 3, 16 and 27, respectively, and anti-e in 10, 21 and 26. Subtypes of HBsAg were similar in carriers with e Ag and with anti-e. There is a high prevalence of e Ag in healthy individuals in Japan. There are also high rates of vertical transmission of hepatitis B virus from mothers to children, as well as a high incidence in the past of post-transfusion hepatitis. This is further evidence that e antigen is a marker for the infectivity of hepatitis B virus in carriers.
采用免疫粘连血凝法对日本4个地区血液中心的292名自愿无偿献血者的血清样本进行了乙肝表面抗原（HBsAg）检测。将他们的血清样本浓缩3倍，并用免疫扩散法检测e抗原（e Ag）和e抗体（抗 - e）。在41份样本（14.0%）中发现了e抗原，在57份样本（18.6%）中发现了抗 - e。当对100份随机选取的含HBsAg血清样本直接检测（未浓缩）以及浓缩3倍和5倍后进行检测时，分别在3份、16份和27份样本中检测到e抗原，在10份、21份和26份样本中检测到抗 - e。携带e抗原和抗 - e的HBsAg亚型相似。在日本健康个体中e抗原的流行率较高。乙肝病毒从母亲到孩子的垂直传播率也很高，以及过去输血后肝炎的发病率也很高。这进一步证明e抗原是乙肝病毒携带者传染性的一个标志物。
