Horwitz C A, Henle W, Henle G, Polesky H, Wexler H, Ward P
Blood. 1976 Jan;47(1):91-8.
Over several years sera were collected from 14 heterophil-positive students or patients who did not fulfill minimal hematologic criteria for infectious mononucleosis (I.M.) The specificity of these heterophil reactions for I.M. was investigated by determining antibodies to Epstein-Barr virus-determined antigens, i.e., to viral capsid antigens (VCA), early antigens (EA), and EBV-associated nuclear antigens (EBNA). On the basis of detectable anti-EA and/or the early absence and late emergence of anti-EBNA, four of these 14 individuals showed evidence of a current or very recent primary Epstein-Barr virus infection. The other ten patients showed antibody patterns indicative of Epstein-Barr virus infections in the past, and no firm conclusions could be drawn with regard to the specificity of their heterophil reactions. It was assumed, however, that some represented atypical clinical forms of EBV infection and that timing of specimen collection was a factor in explaining the paucity of Downey cells. In three patients, the absorbed heterophil-positive reactions persisted with little change in titer for at least 22 mo and thus might represent false-positive tests.
数年间，从14名嗜异性抗体阳性的学生或患者身上采集了血清，这些人不符合传染性单核细胞增多症（I.M.）的最低血液学标准。通过检测针对爱泼斯坦-巴尔病毒（EBV）特定抗原的抗体，即病毒衣壳抗原（VCA）、早期抗原（EA）和EBV相关核抗原（EBNA），研究了这些嗜异性反应对I.M.的特异性。基于可检测到的抗EA和/或抗EBNA早期缺失及后期出现的情况，这14人中的4人显示出当前或近期原发性爱泼斯坦-巴尔病毒感染的证据。其他10名患者显示出过去感染爱泼斯坦-巴尔病毒的抗体模式，关于其嗜异性反应的特异性无法得出确切结论。然而，推测其中一些代表了EBV感染的非典型临床形式，并且标本采集时间是解释唐尼细胞数量稀少的一个因素。在3名患者中，吸收后的嗜异性阳性反应持续至少22个月，滴度变化不大，因此可能代表假阳性检测。
