Zekri A R, Bahnassy A A, Ramadan A S, El-Bassuoni M, Badran A, Madwar M A
Cancer Biology Dept., National Cancer Institute, Cairo University, Egypt.
Infection. 2001 Jan-Feb;29(1):24-6. doi: 10.1007/s15010-001-0010-8.
The RNA genome of hepatitis C virus (HCV) displays extensive sequence variation. In this study, serotyping and genotyping techniques were applied to assess this variability by comparing the performance of the serotyping assay with a panel of well-characterized HCV strains isolated from chronic active hepatitis (CAH) patients.
60 serum samples from CAH patients were analyzed. All isolates were genotyped by a line probe assay and the results of genotyping and serotyping were evaluated.
The overall sensitivity of the serotyping and genotyping techniques was 81.16% with a concordance of 73.3%. Type 4 was detected in 73.3% of cases and it was highly heterogeneous.
Type 4 HCV is the most prevalent type in Egyptian CAH patients and there is a high concordance between the results of serotyping and genotyping techniques.
丙型肝炎病毒（HCV）的RNA基因组表现出广泛的序列变异。在本研究中，通过将血清分型检测的性能与一组从慢性活动性肝炎（CAH）患者中分离出的特征明确的HCV毒株进行比较，应用血清分型和基因分型技术来评估这种变异性。
分析了60例CAH患者的血清样本。所有分离株均通过线性探针检测进行基因分型，并对基因分型和血清分型的结果进行评估。
血清分型和基因分型技术的总体敏感性为81.16%，一致性为73.3%。4型在73.3%的病例中被检测到，且具有高度异质性。
4型HCV是埃及CAH患者中最常见的类型，血清分型和基因分型技术的结果之间具有高度一致性。
