Shahidi F, Chavan U D, Naczk M, Amarowicz R
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.
J Agric Food Chem. 2001 Feb;49(2):926-33. doi: 10.1021/jf0005317.
Beach pea (Lathyrus maritimus L.) cotyledons and hulls were air-classified into different fractions. The crude protein content (%N x 6.25) of samples ranged from 32.8 to 35.3% in cotyledons and 14.7 to 16.8% in hulls. Crude fiber content was higher in hulls fraction 1 (37.13%) and fraction 2 (36.85%) than in cotyledons (2.83, 2.99, and 3.08% in fractions 1, 2, and 3, respectively). Condensed tannins of cotyledons ranged from 5.76 to 6.90% and of hulls ranged from 52.49 to 57.24%, expressed as catechin equivalents. Minerals, namely P, K, and Zn, were higher in cotyledons, but Ca and Mn were more prevalent in hulls. Nonprotein nitrogen was concentrated in hulls, whereas phytic acid was more abundant in the cotyledons. The UV absorption pattern showed that flavonoids were present in fractions (I-III) from hulls separated on Sephadex LH-20. Fraction III from hulls had the highest content of total phenolics and condensed tannins, but no condensed tannins were detected in fractions I and II from hulls. The antioxidant activity of fractions separated on Sephadex LH-20 from hulls and crude extracts in a beta-carotene-linoleate model system was in the order of fraction III > crude extract > fraction II > fraction I. Spots on silica gel TLC plates, sprayed with a solution of beta-carotene and linoleic acid, indicated that many of the individual compounds were antioxidative in nature. Further, separation of fraction III from hulls on a semipreparative HPLC showed the presence of (+) catechin and (-) epicatechin as the main low-molecular-weight phenolic compounds present.
海滩豌豆（海滨山黧豆）的子叶和外壳被气流分级为不同部分。子叶样品的粗蛋白含量（%N×6.25）在32.8%至35.3%之间，外壳的粗蛋白含量在14.7%至16.8%之间。外壳的第1部分（37.13%）和第2部分（36.85%）的粗纤维含量高于子叶（第1、2和3部分分别为2.83%、2.99%和3.08%）。以儿茶素当量表示，子叶的缩合单宁含量在5.76%至6.90%之间，外壳的缩合单宁含量在52.49%至57.24%之间。矿物质，即磷、钾和锌，在子叶中含量较高，但钙和锰在外壳中更为普遍。非蛋白氮集中在外壳中，而植酸在子叶中含量更高。紫外吸收模式表明，在Sephadex LH - 20上分离的外壳部分（I - III）中存在黄酮类化合物。外壳的第III部分总酚类和缩合单宁含量最高，但在外壳的第I和第II部分未检测到缩合单宁。在β-胡萝卜素-亚油酸模型系统中，从外壳和粗提物在Sephadex LH - 20上分离的部分的抗氧化活性顺序为：第III部分＞粗提物＞第II部分＞第I部分。在硅胶TLC板上喷洒β-胡萝卜素和亚油酸溶液后的斑点表明，许多单个化合物具有抗氧化性质。此外，在半制备HPLC上对外壳的第III部分进行分离，结果表明存在（+）儿茶素和（-）表儿茶素作为主要的低分子量酚类化合物。
