Moffitt M C, Neilan B A
School of Microbiology and Immunology, The University of New South Wales, Sydney, N.S.W. 2052, Australia.
FEMS Microbiol Lett. 2001 Mar 15;196(2):207-14. doi: 10.1111/j.1574-6968.2001.tb10566.x.
Nodularin is a hepatotoxin produced by the bloom-forming cyanobacterial species Nodularia spumigena. Putative peptide synthetase and polyketide synthase genes were detected in toxic strains of Nodularia by degenerate PCR. Using specific primer sets, peptide synthetase and polyketide synthase gene homologues were detected in nodularin-producing strains indicating a possible role of peptide synthetase and polyketide synthase enzyme complexes in the biosynthesis of nodularin. Strains of Nodularia isolated from around the world were also analyzed for the production of nodularin by the protein phosphatase 2A inhibition assay. The protein phosphatase inhibition assay and the molecular detection of peptide synthetase and polyketide synthase genes in Nodularia may be useful techniques for the assessment of nodularin-producing cyanobacteria in the environment.
节球藻毒素是由形成水华的蓝藻物种泡沫节球藻产生的一种肝毒素。通过简并PCR在节球藻的有毒菌株中检测到了假定的肽合成酶和聚酮化合物合成酶基因。使用特异性引物对，在产生节球藻毒素的菌株中检测到了肽合成酶和聚酮化合物合成酶基因同源物，这表明肽合成酶和聚酮化合物合成酶酶复合物在节球藻毒素生物合成中可能发挥作用。还通过蛋白磷酸酶2A抑制试验分析了从世界各地分离的节球藻菌株的节球藻毒素产生情况。节球藻中蛋白磷酸酶抑制试验以及肽合成酶和聚酮化合物合成酶基因的分子检测可能是评估环境中产生节球藻毒素的蓝藻的有用技术。
