Integrative Taxonomy and Microbial Ecology Research Group, Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur-741246, Nadia, West Bengal, India.
Centre for Climate and Environmental Studies, Indian Institute of Science Education and Research Kolkata, Mohanpur-741246, Nadia, West Bengal, India.
Arch Microbiol. 2021 May;203(4):1577-1585. doi: 10.1007/s00203-020-02150-0. Epub 2021 Jan 5.
A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem. The isolate has been characterized based on polyphasic approaches. The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements. The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources. Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage). Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites. Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria). The DNA G + C content of the isolate has been determined as 72 mol%. The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose. The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid. Major fatty acids present in the isolate are anteiso-C, iso-C, iso-C, and anteiso-C Whole-genome sequence indicates the size of genome is ~ 5 Mbp. GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700. The isolate I2 has been proposed as a new species, Myceligenerans indicum sp. nov. The genome sequence has been deposited to GenBank/ENA/DDBJ under the accession number JABBYC000000000.
从印度孙德尔本斯红树林生态系统的潮间带沉积物中分离到一株新的放线菌属放线菌种。该分离株是基于多相方法进行特征描述的。该分离株表现出发达的基质菌丝，同时存在球菌和杆状元件。该生物能够在广泛的温度、盐度和 pH 值范围内以及不同的碳源上生长。基于 16S rRNA 的系统发育分析表明，该分离株与 Myceligenerans salitolerans XHU 5031（99%同一性；100%覆盖率）密切相关。在该分离株中存在酮合酶结构域，代表聚酮化合物合酶，这提供了其产生次生代谢物的潜在能力的证据。基于 atpD 和 rpoB 基因序列的多基因系统发育证实，它是放线菌门 Promicromonosporaceae 科内的一个新物种。分离株的 DNA G+C 含量已确定为 72mol%。肽聚糖类型为 A4α，全细胞水解物含有葡萄糖、半乳糖和甘露糖。极性脂类由双磷脂酰甘油、一种未知的磷脂和一种未知的糖脂组成。分离株中存在的主要脂肪酸为 anteiso-C、iso-C、iso-C 和 anteiso-C。全基因组序列表明基因组大小约为 5 Mbp。GGDC（%）、orthoANIu（%）和 I2 基因组的 AAI 分别与 Myceligenerans xiligouense 菌株 DSM 15,700 的基因组的 28.9%、77.44%和 0.859 具有同一性。分离株 I2 被提议为一个新物种，命名为 Myceligenerans indicum sp. nov。该基因组序列已提交给 GenBank/ENA/DDBJ，登录号为 JABBYC000000000。
