Egashira T, Takayama F, Yamanaka Y, Komatsu Y
Department of Pharmacology, Oita Medical University, Hasama-machi, Japan.
Jpn J Pharmacol. 1999 Aug;80(4):379-82. doi: 10.1254/jjp.80.379.
The Kampo medicine Sho-saiko-to scavenged superoxide anion radicals (O2-), hydroxyl radicals (*OH) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent fashion. We attempted to investigate the transition of free radical scavenging activity in plasma after oral administration of Sho-saiko-to in rats. From the response-time profile, kinetic parameters including values for Ka (absorption rate constant), Tmax (peak concentration time), T1/2 (half life) and MRT (mean residence time) of radical scavenging activity in plasma could be calculated for the O2-, *OH and DPPH radicals. These parameters calculated from the dynamics of antioxidation are considered a very meaningful procedure to examine the effects of Sho-saiko-to.
汉方药物小柴胡汤能以剂量依赖方式清除超氧阴离子自由基（O2-）、羟基自由基（*OH）和1,1-二苯基-2-苦基肼（DPPH）自由基。我们试图研究大鼠口服小柴胡汤后血浆中自由基清除活性的变化。根据反应时间曲线，可以计算出血浆中O2-、*OH和DPPH自由基清除活性的动力学参数，包括Ka（吸收速率常数）、Tmax（峰值浓度时间）、T1/2（半衰期）和MRT（平均驻留时间）。从抗氧化动力学计算得到的这些参数被认为是检验小柴胡汤效果的非常有意义的方法。
