Steinman R M, Adams J C, Cohn Z A
J Exp Med. 1975 Apr 1;141(4):804-20.
White pulp nodules of mouse spleen contain a minor population of cells with morphologic features that are identical to those of dendritic cells, a cell type recently described in vitro. They have characteristic large, irregularly shaped nuclei with distinctive chromatin patterns and small nucleoli. The cytoplasm is extended in processes that contain relatively few organelles. These presumptive dendritic cells can be distinguished from other cell types that are known to exist in spleen including those that have irregular or branching cell shapes. In particular, dendritic cells do not contain the large number of lysosomes seen in phagocytes, and do not actively interiorize intravenously administered colloidal thorium dioxide particles. They also lack the well developed secretory apparatus (rough endoplasmic reticulum and Golgi zone) and microfilament bundles that are noted in connective tissue cells. These morphologic observations, combined with previous in vitro work, substantiate the existence of a novel class of cells in mouse lymphoid organs.
小鼠脾脏的白髓小结包含一小部分细胞，其形态特征与树突状细胞相同，树突状细胞是最近在体外描述的一种细胞类型。它们具有特征性的大的、形状不规则的细胞核，具有独特的染色质模式和小核仁。细胞质延伸成含有相对较少细胞器的突起。这些推测的树突状细胞可以与已知存在于脾脏中的其他细胞类型区分开来，包括那些具有不规则或分支细胞形状的细胞。特别是，树突状细胞不含吞噬细胞中可见的大量溶酶体，也不会主动内化静脉注射的二氧化钍胶体颗粒。它们也缺乏结缔组织细胞中可见的发达的分泌装置（粗面内质网和高尔基体区）和微丝束。这些形态学观察结果，结合先前的体外研究工作，证实了小鼠淋巴器官中存在一类新型细胞。
