在酿酒酵母中进行的大规模过表达筛选鉴定出了以前未被表征的细胞周期基因。
A large-scale overexpression screen in Saccharomyces cerevisiae identifies previously uncharacterized cell cycle genes.
作者信息
Stevenson L F, Kennedy B K, Harlow E
机构信息
Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3946-51. doi: 10.1073/pnas.051013498.
Abstract
We have undertaken an extensive screen to identify Saccharomyces cerevisiae genes whose products are involved in cell cycle progression. We report the identification of 113 genes, including 19 hypothetical ORFs, which confer arrest or delay in specific compartments of the cell cycle when overexpressed. The collection of genes identified by this screen overlaps with those identified in loss-of-function cdc screens but also includes genes whose products have not previously been implicated in cell cycle control. Through analysis of strains lacking these hypothetical ORFs, we have identified a variety of new CDC and checkpoint genes.
摘要
我们进行了一项广泛的筛选，以鉴定酿酒酵母中其产物参与细胞周期进程的基因。我们报告鉴定出113个基因，包括19个假定的开放阅读框，当这些基因过表达时，会导致细胞周期特定阶段的停滞或延迟。通过该筛选鉴定出的基因集合与在功能丧失型cdc筛选中鉴定出的基因有重叠，但也包括其产物以前未涉及细胞周期调控的基因。通过对缺乏这些假定开放阅读框的菌株进行分析，我们鉴定出了多种新的CDC和检查点基因。
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