Cho Y J, Chang M S, Park S H, Kim H S, Kim W H
Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
Hum Pathol. 2001 Mar;32(3):297-301. doi: 10.1053/hupa.2001.22766.
Unlike gastric carcinoma, associations of the Epstein-Barr virus (EBV) with carcinomas of other sites in the gastrointestinal tract have not yet been clarified. To elucidate these associations, we investigated the presence of EBV in 142 cases of esophageal carcinoma, 107 cases of ampulla of Vater carcinoma, and 274 cases of colorectal carcinoma in Korean patients using EBV-encoded small RNAs (EBER)-in situ hybridization (ISH). In all cases, none of the tumor cells showed a positive signal, indicating that EBV is not generally related to the carcinogenesis of these cancers. Some EBV-positive tumor-infiltrating lymphocytes (TILs) were found in 8 of 142 cases (5.6%) of esophageal carcinoma, 8 of 107 cases (7.5%) of ampulla of Vater cancer, and 35 of 274 cases (12.8%) of colorectal carcinoma. For comparison, EBER-ISH was performed in consecutive gastric carcinomas; the EBER signal on tumor cells was observed in 17 of 306 cases (5.6%), and EBV-positive TILs were seen in 31 of the 289 cases (10.7%). There was no statistically significant difference in the frequencies of cases with EBV-positive TILs among the gastrointestinal tract cancers. We suggest that the reservoir lymphocytes carrying EBV, like other inflammatory cells, are able to reach anywhere, and that the chance for an epithelial cell to be exposed to EBV is similar at different sites of the gastrointestinal tract, regardless of its carcinogenic effect on the epithelial cell. HUM PATHOL 32:297-301.
与胃癌不同，爱泼斯坦-巴尔病毒（EBV）与胃肠道其他部位癌症的关联尚未明确。为阐明这些关联，我们采用EBV编码的小RNA（EBER）原位杂交（ISH）技术，对142例韩国食管癌患者、107例壶腹癌患者和274例结直肠癌患者的EBV存在情况进行了研究。在所有病例中，肿瘤细胞均未显示阳性信号，这表明EBV通常与这些癌症的发生无关。在142例食管癌中有8例（5.6%）、107例壶腹癌中有8例（7.5%）以及274例结直肠癌中有35例（12.8%）发现了一些EBV阳性的肿瘤浸润淋巴细胞（TIL）。作为对照，我们对连续的胃癌病例进行了EBER-ISH检测；在306例胃癌中有17例（5.6%）观察到肿瘤细胞上有EBER信号，在289例中有31例（10.7%）发现了EBV阳性的TIL。胃肠道癌症中EBV阳性TIL病例的频率没有统计学上的显著差异。我们认为，携带EBV的储备淋巴细胞与其他炎症细胞一样，能够到达任何部位，并且上皮细胞暴露于EBV的机会在胃肠道的不同部位是相似的，无论其对上皮细胞是否具有致癌作用。《人类病理学》32:297 - 301。
