Mense M G, Van De Verg L L, Bhattacharjee A K, Garrett J L, Hart J A, Lindler L E, Hadfield T L, Hoover D L
Department of Diagnostic Pathology, Walter Reed Army Institute of Research, Silver Spring, MD 20910-7500, USA.
Am J Vet Res. 2001 Mar;62(3):398-405. doi: 10.2460/ajvr.2001.62.398.
To characterize effects of intranasal inoculation of virulent Brucella melitensis strain 16M in mice.
Female Balb/c mice, 6 to 8 weeks old.
Studies were designed to elucidate gross morphologic lesions, bacterial burden in target organs, and histologic changes in tissues following experimental intranasal inoculation of mice with B melitensis 16M, which could be used to characterize a model for testing vaccine efficacy.
Measurable splenomegaly was evident at 3 and 7 weeks after inoculation. A demonstrable increase in splenic colony-forming units (CFU) from infected mice increased over time with increasing dose when comparing inocula of 10(3), 10(4), and 10(5) CFU. Recovery of brucellae from the lungs was possible early in infection with 10(1), 10(3), and 10(5) CFU, but only the group inoculated with 10(5) CFU consistently yielded quantifiable bacteria. At a dose of 10 CFU, few organisms were located in the spleen. Bacteria were recovered up to 140 days after inoculation in mice given 10(3) CFU. At an inoculum of 10(5) CFU, bacterial counts were highest early in infection. Histologic examination of tissues revealed an increase in white pulp and marginal zone in the spleen and lymphohistiocytic hepatitis.
Changes in the spleen and liver increased with increases in dose and with increased time following intranasal inoculation with B melitensis 16M. Surprisingly, histologic changes were not observed in the lungs of inoculated mice.
描述鼻内接种强毒力羊种布鲁氏菌16M菌株对小鼠的影响。
6至8周龄雌性Balb/c小鼠。
设计实验以阐明实验性鼻内接种羊种布鲁氏菌16M后小鼠的大体形态学损伤、靶器官中的细菌载量以及组织学变化，这些可用于表征一种测试疫苗效力的模型。
接种后3周和7周时可测量到脾脏肿大明显。比较接种10³、10⁴和10⁵CFU菌液时，感染小鼠脾脏中的集落形成单位（CFU）随时间和剂量增加而明显增加。感染初期，接种10¹、10³和10⁵CFU菌液后可从肺中分离出布鲁氏菌，但只有接种10⁵CFU菌液组始终能分离出可定量的细菌。接种剂量为10CFU时，脾脏中几乎没有细菌。接种10³CFU菌液的小鼠在接种后140天仍可分离出细菌。接种10⁵CFU菌液时，感染初期细菌数量最高。组织学检查显示脾脏白髓和边缘区增加以及淋巴细胞性肝炎。
鼻内接种羊种布鲁氏菌16M后，脾脏和肝脏的变化随剂量增加和时间延长而增加。令人惊讶的是，接种小鼠的肺部未观察到组织学变化。
