Battelle, 505 King Avenue Columbus, Ohio 43201, USA.
Sci Rep. 2012;2:495. doi: 10.1038/srep00495. Epub 2012 Jul 6.
To characterize the clinical presentation and pathophysiology of inhalational brucellosis, Balb/c mice were challenged with Brucella melitensis 16M in a nose-only aerosol exposure chamber. A low dose of 1000 cfu/animal of B. melitensis resulted in 45% of mice with tissue burdens eight weeks post-challenge. The natural history of brucellosis in mice challenged by higher aerosol doses was examined by serial euthanizing mice over an eight week period. Higher challenge doses of 1.00E+05 and 5.00E+05 cfu resulted in positive blood cultures 14 days post-challenge and bacterial burdens were observed in the lung, liver and/or spleens 14 days post-challenge. In addition, the progression of brucellosis was similar between mice challenged by the intranasal and aerosol routes. The results from this study support the use of the Balb/c aerosol nose-only brucellosis mouse model for the evaluation of therapeutics against inhalational brucellosis.
为了描述吸入性布鲁氏菌病的临床特征和病理生理学，我们将 Balb/c 小鼠置于鼻内仅暴露于气溶胶的染毒室中，用布鲁氏菌 melitensis 16M 进行挑战。低剂量 1000cfu/动物的布鲁氏菌 melitensis 导致 45%的小鼠在挑战后 8 周时具有组织负担。通过在 8 周的时间内连续对小鼠进行安乐死，检查了更高气溶胶剂量感染的小鼠中布鲁氏菌病的自然史。更高的挑战剂量 1.00E+05 和 5.00E+05cfu 导致 14 天攻毒后血培养阳性，14 天攻毒后在肺、肝和/或脾中观察到细菌负担。此外，鼻内和气溶胶途径感染的小鼠之间的布鲁氏菌病进展相似。这项研究的结果支持使用 Balb/c 气溶胶鼻内布鲁氏菌病小鼠模型来评估针对吸入性布鲁氏菌病的治疗方法。
