Olsen S C, Waters W R, Stoffregen W S
Bacterial Diseases Research Unit, National Animal Disease Center, USDA, Agriculture, Research Service, Ames, IA 50010, USA.
Zoonoses Public Health. 2007;54(8):281-5. doi: 10.1111/j.1863-2378.2007.01063.x.
To characterize the optimal aerosol dosage of Brucella abortus strain 2308 (S2308) and B. melitensis (S16M) in a laboratory animal model of brucellosis, dosages of 10(3)-10(10) colony forming units (CFU) were nebulized to mice. Although tissue weights were minimally influenced, total CFU per tissues increased beginning at 10(6)-10(7) CFU dosages, with 10(9) CFU appearing to be an optimal dosage for S16M or S2308 aerosol delivery. At 12 weeks after vaccination with 10(7) CFU of B. abortus strain RB51 (SRB51) or saline (control), mice were challenged intraperitoneally (i.p.) (6.4 x 10(4) CFU) or via aerosol (1.76 x 10(9) CFU) with S2308. Mice vaccinated with SRB51 had reduced (P < 0.05) splenic, liver and lung colonization (total CFU and CFU/g) after i.p. challenge with S2308 as compared with control mice after i.p. S2308 challenge. Control and SRB51-vaccinated mice did not differ (P > 0.05) in splenic, liver or lung colonization after aerosol S2308 challenge. Failure to demonstrate vaccine protection was not because of a high aerosol challenge dosage as colonization of spleen and liver tissues was lower (P < 0.05) after aerosol challenge when compared with control mice after i.p. S2308 challenge.
为了在布鲁氏菌病实验动物模型中确定流产布鲁氏菌2308菌株（S2308）和马尔他布鲁氏菌（S16M）的最佳气溶胶剂量，将10³-10¹⁰菌落形成单位（CFU）的剂量雾化给小鼠。尽管组织重量受到的影响最小，但从10⁶-10⁷CFU剂量开始，每个组织的总CFU增加，10⁹CFU似乎是S16M或S2308气溶胶递送的最佳剂量。在用10⁷CFU的流产布鲁氏菌RB51菌株（SRB51）或生理盐水（对照）接种疫苗12周后，小鼠经腹腔注射（i.p.）（6.4×10⁴CFU）或通过气溶胶（1.76×10⁹CFU）用S2308进行攻击。与经腹腔注射S2308攻击后的对照小鼠相比，用SRB5接种疫苗的小鼠在经腹腔注射S2308攻击后脾脏、肝脏和肺部的定植（总CFU和CFU/g）减少（P<0.05）。在经气溶胶S2308攻击后，对照小鼠和接种SRB51的小鼠在脾脏、肝脏或肺部的定植方面没有差异（P>0.05）。未能证明疫苗保护作用并非由于气溶胶攻击剂量过高，因为与经腹腔注射S2308攻击后的对照小鼠相比，气溶胶攻击后脾脏和肝脏组织的定植较低（P<0.05）。
