韩国梅久中黄曲霉毒素的自然存在情况。

Natural occurrence of aflatoxins in Korean meju.

作者信息

Kim E K, Shon D H, Yoo J Y, Ryu D, Lee C, Kim Y B

机构信息

The Graduate School of Biotechnology, Korea University, Seoul.

出版信息

Food Addit Contam. 2001 Feb;18(2):151-6. doi: 10.1080/02652030010006104.

DOI:10.1080/02652030010006104

PMID:11288912

Abstract

Aflatoxin B1 (AFB1) was found in 35 of 60 (58.3%) meju samples with an average concentration of 7.3 ng/g by ELISA. Contamination of AFB1 was confirmed in 25 of 60 samples (41.6%) using HPLC, with an average concentration of 6.9 ng/g. Mean recoveries from meju ranged from 107% to 170% for AFB1 using ELISA at a spiking range of 1 to 50 ng/g. Over the same range, recoveries using HPLC were from 70% to 83%. The levels of AFB1 determined by ELISA and by HPLC demonstrated a close relationship between the two methods (r2 = 0.9324) employed in this study. In order to evaluate the potential health risks of AFB1 on Koreans consuming meju, we calculated the estimated probable daily intake (PDI) based on the average contamination levels and compared it with the estimated tolerable daily intake (TDI). The PDIs of AFB1 from kanjang and dwenjang were determined to be 0.04 and 0.21 ng/kg bw/day, respectively, and were higher than TDIs.

摘要

通过酶联免疫吸附测定法（ELISA），在60份豆酱样品中的35份（58.3%）中检测到黄曲霉毒素B1（AFB1），平均浓度为7.3纳克/克。使用高效液相色谱法（HPLC）在60份样品中的25份（41.6%）中确认了AFB1污染，平均浓度为6.9纳克/克。在1至50纳克/克的加标范围内，使用ELISA法对豆酱中AFB1的平均回收率为107%至170%。在相同范围内，使用HPLC法的回收率为70%至83%。本研究中采用的ELISA法和HPLC法测定的AFB1水平显示出两种方法之间的密切关系（r2 = 0.9324）。为了评估AFB1对食用豆酱的韩国人潜在的健康风险，我们根据平均污染水平计算了估计的每日可能摄入量（PDI），并将其与估计的每日可耐受摄入量（TDI）进行比较。酱油和大酱中AFB1的PDI分别确定为0.04和0.21纳克/千克体重/天，均高于TDI。