Vrbanac J J, Tilson H A, Moore K E, Rech R H
Pharmacol Biochem Behav. 1975 Jan-Feb;3(1):57-64. doi: 10.1016/0091-3057(75)90080-5.
The neurochemical effects of DOM and d-amphetamine were compared under several conditions in unanesthetized rats implanted with chronic-indwelling push-pull cannulae in a cerebral lateral ventricle. Brain catecholamine storage sites were previously pulse-labelled with 14-C-norepinephrine administered intraventricularly. During the perfusion of the lateral ventricles with artificial cerebrospinal fluid, the animals were injected i.p. with 1.5 mg/kg of DOM, 2.0 mg/kg of d-amphetamine or 1.0 ml/kg of isotonic saline. Analysis of the perfusate in successive samples indicated an increased efflux of 14-C-radioactivity in rats administered DOM or d-amphetamine. Increased proportions of 14-C-norepinephrine and 14-C-normetanephrine were detected in samples of perfusate 15 to 30 min after drug injection. Pretreatment of other animals with 6-hydroxydopamine intraventricularly, which decreased brain levels of both norepinephrine and dopamine, blocked the increased efflux of 14-C-radioactivity induced by DOM or d-amphetamine. Pretreatment of rats with 6-hydroxydopa i.p., which depleted brain norepinephrine selectively, reduced to about half the d-amphetamine-induced efflux of 14-C-radioactivity for all samples during the time course of the effect. However, animals pretreated with 6-hydroxydopa and then tested for DOM effects showed a different pattern of 14-C-radioactivity efflux. The efflux for the initial samples was increased as with the DOM control, but the 6-hydroxydopa pretreatment attuated the DOM-induced efflux for the later samples. The results suggest DOM and d-amphetamine share qualitatively similar effects in releasing and/or blocking the reuptake of catecholamines at brain periventricular nerve terminals. Nevertheless, DOM appears to differ from d-amphetamine in the temporal pattern of net catecholamine release.
在几种条件下，对未麻醉的、在大脑侧脑室植入慢性留置推挽式套管的大鼠，比较了DOM和d-苯丙胺的神经化学效应。脑儿茶酚胺储存部位先前已通过脑室内注射14-C-去甲肾上腺素进行脉冲标记。在用人工脑脊液灌注侧脑室期间，给动物腹腔注射1.5毫克/千克的DOM、2.0毫克/千克的d-苯丙胺或1.0毫升/千克的等渗盐水。对连续样本中的灌流液进行分析表明，给予DOM或d-苯丙胺的大鼠中14-C放射性的流出增加。在注射药物后15至30分钟的灌流液样本中，检测到14-C-去甲肾上腺素和14-C-去甲变肾上腺素的比例增加。用6-羟基多巴胺脑室内预处理其他动物，降低了去甲肾上腺素和多巴胺的脑水平，阻断了DOM或d-苯丙胺诱导的14-C放射性流出增加。用6-羟基多巴腹腔预处理大鼠，选择性地耗尽脑去甲肾上腺素，在效应的时间进程中，所有样本中d-苯丙胺诱导的14-C放射性流出减少到大约一半。然而，用6-羟基多巴预处理然后测试DOM效应的动物显示出不同的14-C放射性流出模式。初始样本的流出与DOM对照组一样增加，但6-羟基多巴预处理减弱了后期样本中DOM诱导的流出。结果表明，DOM和d-苯丙胺在释放和/或阻断脑室周围神经末梢的儿茶酚胺再摄取方面具有定性相似的效应。然而，DOM在儿茶酚胺净释放的时间模式上似乎与d-苯丙胺不同。
