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布兰奇福尔蒂·巴雷西王子家族遗骸的DNA分析。

DNA analyses of the remains of the Prince Branciforte Barresi family.

作者信息

Rickards O, Martínez-Labarga C, Favaro M, Frezza D, Mallegni F

机构信息

Dipartimento di Biologia, Università di Roma "Tor Vergata", Via della Ricerca Scientifica n. 1, Roma, Italia.

出版信息

Int J Legal Med. 2001;114(3):141-6. doi: 10.1007/s004149900119.

DOI:10.1007/s004149900119
PMID:11296885
Abstract

The five skeletons found buried in the church of Militello di Catania, Sicily, were tentatively identified by morphological analysis and historical reports as the remains of Prince Branciforte Barresi, two of his children, his brother and another juvenile member of the family (sixteenth and seventeenth centuries). In order to attempt to clarify the degree of relationships of the five skeletons, sex testing and mitochondrial DNA (mtDNA) sequence analysis of the hypervariable segments I and II (HV1 and HV2) of control region were performed. Moreover, the 9 bp-deletion marker of region V (COII/tRNAlys) was examined. Molecular genetic analyses were consistent with historical expectations, although they did not directly demonstrate that these are in fact the remains of the Prince and his relatives, due to the impossibility of obtaining DNA from living maternal relatives of the Prince.

摘要

在西西里岛卡塔尼亚的米利泰洛教堂发现的五具骸骨,通过形态分析和历史报告初步鉴定为布兰卡福尔泰·巴雷西王子、他的两个孩子、他的兄弟以及家族中的另一名青少年成员(16和17世纪)的遗骸。为了试图厘清这五具骸骨之间的亲属关系程度,对控制区高变区I和II(HV1和HV2)进行了性别检测和线粒体DNA(mtDNA)序列分析。此外,还检测了区域V(COII/tRNAlys)的9碱基缺失标记。分子遗传学分析与历史预期相符,不过由于无法从王子在世的母系亲属那里获取DNA,所以这些分析并未直接证明这些骸骨实际上就是王子及其亲属的遗骸。

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