Löffler H, Hoffmann R, Happle R, Effendy I
Department of Dermatology, Philipp University, Marburg, Germany.
Clin Exp Dermatol. 2001 Mar;26(2):196-200. doi: 10.1046/j.1365-2230.2001.00795.x.
The standard method for evaluating contact allergy in mice is the ear swelling technique. However, in experimental irritant contact dermatitis, the epidermal barrier disruption, that represents a predominant effect of irritants, cannot be assayed by this
An appropriate method to evaluate barrier disruption is the measurement of transepidermal water loss (TEWL) but to date this has so far been possible only on the trunk of hairless or shaved mice. We therefore developed a new technique to measure the TEWL of mice ears (murine auricular TEWL: MATEWL). After patch testing with irritants and allergens, respectively, we found that the ear swelling method is most suitable for evaluating allergic skin reactions, whereas MATEWL is most appropriate for evaluating irritant skin reactions.
评估小鼠接触性过敏的标准方法是耳部肿胀技术。然而，在实验性刺激性接触性皮炎中，代表刺激物主要作用的表皮屏障破坏无法通过该方法进行检测。
评估屏障破坏的一种合适方法是测量经表皮水分流失（TEWL），但迄今为止，这仅在无毛或剃毛小鼠的躯干上可行。因此，我们开发了一种测量小鼠耳部TEWL的新技术（小鼠耳廓TEWL：MATEWL）。在用刺激物和变应原分别进行斑贴试验后，我们发现耳部肿胀方法最适合评估过敏性皮肤反应，而MATEWL最适合评估刺激性皮肤反应。
