耐盐酵母汉逊德巴利酵母中两个编码钠泵的基因的克隆与表达。
Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii.
作者信息
Almagro A, Prista C, Benito B, Loureiro-Dias M C, Ramos J
机构信息
Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, E-14071 Córdoba, Spain.
出版信息
J Bacteriol. 2001 May;183(10):3251-5. doi: 10.1128/JB.183.10.3251-3255.2001.
Abstract
Two genes encoding Na(+)-ATPases from Debaryomyces hansenii were cloned and sequenced. The genes, designated ENA1 from D. hansenii (DhENA1) and DhENA2, exhibited high homology with the corresponding genes from Schwanniomyces occidentalis. DhENA1 was expressed in the presence of high Na(+) concentrations, while the expression of DhENA2 also required high pH. A mutant of Saccharomyces cerevisiae lacking the Na(+) efflux systems and sensitive to Na(+), when transformed with DhENA1 or DhENA2, recovered Na(+) tolerance and also the ability to extrude Na(+).
摘要
克隆并测序了来自汉逊德巴利酵母的两个编码Na(+)-ATP酶的基因。这两个基因，分别命名为来自汉逊德巴利酵母的ENA1（DhENA1）和DhENA2，与西方许旺酵母的相应基因具有高度同源性。DhENA1在高Na(+)浓度下表达，而DhENA2的表达也需要高pH值。缺乏Na(+)外排系统且对Na(+)敏感的酿酒酵母突变体，在用DhENA1或DhENA2转化后，恢复了对Na(+)的耐受性以及排出Na(+)的能力。
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