Walker R, Innes C M, Allan E J
Department of Agriculture & Forestry, University of Aberdeen, UK.
Lett Appl Microbiol. 2001 May;32(5):346-8. doi: 10.1046/j.1472-765x.2001.00915.x.
Antifungal metabolites of Pseudomonas antimicrobica have previously been shown to inhibit conidial germination of the grey mould pathogen Botrytis cinerea. In this study, metabolites of the bacterium have been tested at different stages of Botrytis germination to determine their effects on germ tube production and extension.
Metabolites were added to conidia that had been pre-incubated for either 120 or 255 min. Pseudomonas antimicrobica inhibited B. cinerea conidial germination and caused a significant reduction in germ tube extension, irrespective of the stage of germination. Abnormal germination and a reduction in the frequency of lateral branching of the germ tubes in the presence of the metabolites were also reported, suggesting interference with normal hyphal development.
The bacterium can inhibit germination of conidia and extension of germ tubes at different stages of Botrytis development.
The antagonistic activity of the bacterium has promising implications for its use as a biocontrol agent.
先前已表明抗微生物假单胞菌的抗真菌代谢产物可抑制灰霉病菌灰葡萄孢的分生孢子萌发。在本研究中，已在灰葡萄孢萌发的不同阶段测试了该细菌的代谢产物，以确定它们对芽管产生和延伸的影响。
将代谢产物添加到已预孵育120或255分钟的分生孢子中。无论萌发阶段如何，抗微生物假单胞菌均抑制灰葡萄孢分生孢子萌发，并导致芽管延伸显著减少。还报道了在代谢产物存在下异常萌发以及芽管侧枝频率降低，这表明对正常菌丝发育存在干扰。
该细菌可抑制灰葡萄孢发育不同阶段的分生孢子萌发和芽管延伸。
该细菌的拮抗活性对其作为生物防治剂的应用具有广阔前景。
