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微波辅助组织处理、不同厚度组织的固定和染色：一项比较研究。

Microwave-assisted tissue processing, fixation and staining in tissues of different thicknesses: A comparative study.

作者信息

Rao Mahesh, Pai Shaila M, Khanagar Sanjeev Balappa, Siddeeqh Salman, Devang Darshan Divakar, Naik Sachin

机构信息

Department of Oral Pathology and Microbiology, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, India.

Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia, Karnataka, India.

出版信息

J Oral Maxillofac Pathol. 2020 Jan-Apr;24(1):186. doi: 10.4103/jomfp.JOMFP_290_19. Epub 2020 May 8.

DOI:10.4103/jomfp.JOMFP_290_19
PMID:32508477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7269310/
Abstract

AIM AND OBJECTIVES

The study aimed at assessment of microwave assisted tissue fixation, processing and staining and to determine if it can replace standard formalin fixed paraffin embedded processing in tissues of different thickness.

MATERIALS AND METHODS

Specimens from buccal mucosa and gingiva was used in the study and were divided into three different thickness and was fixed, processed and stained according to conventional method and with a use of kitchen microwave oven respectively. The present study is the first of its kind where oral tissues was fixed, processed and stained with a kitchen microwave in three different thickness. The results obtained was statistically analyzed using IBM SPSS Statistics version 21.0 software.

RESULTS

The new technique of fixation, tissue processing and staining using a microwave employed in the present study represented a major change from conventional method and achieved significant reduction in time taken.

CONCLUSION

The ease of application and speed of this technique significantly reduced turnaround time in diagnostic labs.

摘要

目的

本研究旨在评估微波辅助组织固定、处理和染色，并确定其是否能在不同厚度的组织中替代标准的福尔马林固定石蜡包埋处理方法。

材料与方法

本研究使用来自颊黏膜和牙龈的标本，将其分为三种不同厚度，并分别按照传统方法以及使用家用微波炉进行固定、处理和染色。本研究是首次对三种不同厚度的口腔组织使用家用微波炉进行固定、处理和染色。使用IBM SPSS Statistics 21.0软件对所得结果进行统计学分析。

结果

本研究中采用的使用微波进行固定、组织处理和染色的新技术与传统方法相比有重大改变，并显著缩短了所需时间。

结论

该技术的易用性和速度显著缩短了诊断实验室的周转时间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/5790307ef0b8/JOMFP-24-186b-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/1102e5b7ed8c/JOMFP-24-186b-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/6b71c1849c6b/JOMFP-24-186b-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/805edc58ec2f/JOMFP-24-186b-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/373536ae7236/JOMFP-24-186b-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/5790307ef0b8/JOMFP-24-186b-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/1102e5b7ed8c/JOMFP-24-186b-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/6b71c1849c6b/JOMFP-24-186b-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/805edc58ec2f/JOMFP-24-186b-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/373536ae7236/JOMFP-24-186b-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8714/7269310/5790307ef0b8/JOMFP-24-186b-g005.jpg

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