Pert J H, Verch R L
Vox Sang. 1975;29(3):157-76. doi: 10.1111/j.1423-0410.1975.tb00491.x.
An immune agglutination procedure is described in which red cells are coated with antigen and agglutination is observed after a short incubation with antibody in reagent serum. Conversely, the cells may be coated with antibody and used to test for the presence of antigen. Treatment of the reagent serum with cobra venom factor results in inhibition, and there is a marked lo-s of sensitivity with cells pretreated with formalin or chromic chloride, suggesting that C3 and some type of reactive sites on the cells are required. The distinctive features of the new procedure are that incubation time is very short, sensitivity is several orders of magnitude higher than with other related tests, and, within some limitations, bovine (steer) red cells with bovine serum can be used as an alternative to human red cells with human serum. It is necessary to remove an inhibitor from the reagent serum, human or steer. While the mechanism of the procedure needs further elucidation, the findings reported here suggest that it ought to be sufficiently sensitive to detect hepatitus B antigen if present in virtually any blood sample. With appropriate modifications it should be useful for blood donor screening, diagnosis, and for studying many other antigen-antibody systems.
本文描述了一种免疫凝集程序，其中红细胞被抗原包被，在与试剂血清中的抗体短暂孵育后观察凝集现象。相反，细胞也可以被抗体包被并用于检测抗原的存在。用眼镜蛇毒因子处理试剂血清会导致抑制作用，并且用福尔马林或氯化铬预处理的细胞敏感性显著降低，这表明需要C3和细胞上某种类型的反应位点。新程序的独特之处在于孵育时间非常短，灵敏度比其他相关测试高几个数量级，并且在某些限制范围内，牛（公牛）红细胞与牛血清可作为人红细胞与人血清的替代品。有必要从人或牛的试剂血清中去除一种抑制剂。虽然该程序的机制需要进一步阐明，但此处报道的结果表明，如果几乎任何血液样本中存在乙肝抗原，它应该有足够的灵敏度来检测。经过适当修改，它应该可用于献血者筛查、诊断以及研究许多其他抗原-抗体系统。
