Chicot D, Wright R, Vnek J, Neurath R, Prince A M
Rev Fr Transfus Immunohematol. 1975 Jun;18(2):137-53. doi: 10.1016/s0338-4535(75)80014-6.
In this report we present an evaluation of the sensitivity, specificity and ability to detect HBs Ag carriers of a new reversed passive hemagglutination test, using immunochemically purified chimpanzee anti HBs bound to stabilized human erythrocytes. The method was shown to have a sensitivity equal (within one two fold dilution) to that of the Ausria I 125 ratio immuno assay, and in a double blind comparison detected essentially the same number of Hbs Ag containing specimens among volunteer blood donors. The method therefore provides an economical method for the third generation testing of blood donors. The methodology which has been described incorporates a definitive specificity test in which serum drawn before and after immunization of chimpanzees with purified HBs Ag is compared for its ability to neutralize the hemagglutination reaction. The use of serum from the same animal for this purpose avoids the theoretical possibility that antiglobulin antibodies directed at subclass determinants such as Gm of Inv could be differentially inhibited due to possible subclass differences in the blocking sera employed. A reliable test for specificity of HBs Ag screening results is essential to avoid false notification of donors that they are carriers of hepatitis B virus.
在本报告中，我们对一种新的反向被动血凝试验进行了敏感性、特异性及检测乙肝表面抗原（HBs Ag）携带者能力的评估。该试验采用免疫化学方法纯化的黑猩猩抗HBs，结合到稳定的人红细胞上。结果表明，该方法的敏感性（在1至2倍稀释范围内）与Ausria I 125比例免疫分析相当，并且在双盲比较中，检测出志愿献血者中含HBs Ag标本的数量基本相同。因此，该方法为献血者的第三代检测提供了一种经济的方法。所描述的方法包含一个明确的特异性试验，即将用纯化的HBs Ag免疫黑猩猩前后抽取的血清进行比较，看其中和血凝反应的能力。为此使用同一动物的血清可避免理论上的可能性，即由于所采用的封闭血清中可能存在的亚类差异，针对如Inv的Gm等亚类决定簇的抗球蛋白抗体可能会受到不同程度的抑制。对于HBs Ag筛查结果的特异性进行可靠检测对于避免错误告知献血者他们是乙肝病毒携带者至关重要。
