Sewell M M, Sherman B K, Neale D B
Institute of Forest Genetics, USDA Forest Service, Pacific Southwest Research Station, Placerville, California 95667, USA.
Genetics. 1999 Jan;151(1):321-30. doi: 10.1093/genetics/151.1.321.
A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree were recoded to reflect the two independent populations of parental meioses, and genetic maps were constructed to represent each parent. The rate of meiotic recombination was significantly greater for males than females, as was the average estimate of genome length for males (1983.7 cM [Kosambi mapping function (K)]) and females [1339.5 cM(K)]. The integration of individual maps allows for the synthesis of genetic information from independent sources onto a single consensus map and facilitates the consolidation of linkage groups to represent the chromosomes n = 12 of loblolly pine. The resulting consensus map consists of 357 unique molecular markers and covers approximately 1300 cM(K).
通过整合来自两个不相关的三代远交谱系的连锁数据，构建了火炬松（Pinus taeda L.）的整合图谱。对每个谱系中限制性片段长度多态性、随机扩增多态性DNA和同工酶遗传标记的子代分离数据进行重新编码，以反映亲本减数分裂的两个独立群体，并构建遗传图谱来代表每个亲本。雄性减数分裂重组率显著高于雌性，雄性基因组长度的平均估计值（1983.7厘摩[科斯ambi作图函数（K）]）和雌性（1339.5厘摩[K]）也是如此。个体图谱的整合使得能够将来自独立来源的遗传信息综合到一个单一的整合图谱上，并有助于整合连锁群以代表火炬松n = 12条染色体。所得的整合图谱由357个独特的分子标记组成，覆盖约1300厘摩（K）。
