使用基于细胞的生物传感器检测生理活性化合物。

Detection of physiologically active compounds using cell-based biosensors.

作者信息

Stenger D A, Gross G W, Keefer E W, Shaffer K M, Andreadis J D, Ma W, Pancrazio J J

机构信息

Center for BioMolecular Science and Engineering, Code 6910, Naval Research Laboratory, Washington, DC 20375, USA.

出版信息

Trends Biotechnol. 2001 Aug;19(8):304-9. doi: 10.1016/s0167-7799(01)01690-0.

DOI:10.1016/s0167-7799(01)01690-0

PMID:11451472

Abstract

Cell-based biosensors are portable devices that contain living biological cells that monitor physiological changes induced by exposure to environmental perturbations such as toxicants, pathogens or other agents. Methods of detecting physiological changes include extracellular electrical recordings, optical measurements, and, in the future, functional genomics and proteomics. Several technical developments are occurring that will increase the feasibility of cell-based biosensors for field applications; these developments include stem cell and 3D culture technologies. Possible scenarios for the use of cell-based biosensors include broad-range detectors of unknown threat agents and functional assessment of identified agents.

摘要

基于细胞的生物传感器是一种便携式设备，其中包含活的生物细胞，可监测因接触环境干扰物（如有毒物质、病原体或其他因子）而引起的生理变化。检测生理变化的方法包括细胞外电记录、光学测量，以及未来的功能基因组学和蛋白质组学。目前正在出现一些技术进展，这将提高基于细胞的生物传感器在现场应用中的可行性；这些进展包括干细胞和三维培养技术。基于细胞的生物传感器的可能应用场景包括未知威胁因子的广谱探测器以及已识别因子的功能评估。