一例对啤酒过敏且显示脂质转移蛋白间存在交叉反应的病例。

A case of allergy to beer showing cross-reactivity between lipid transfer proteins.

作者信息

Asero R, Mistrello G, Roncarolo D, Amato S, van Ree R

机构信息

Ambulatorio di Allergologia, Ospedale Caduti Bollatesi, Bollate, MI, Italy.

出版信息

Ann Allergy Asthma Immunol. 2001 Jul;87(1):65-7. doi: 10.1016/S1081-1206(10)62325-3.

DOI:10.1016/S1081-1206(10)62325-3

PMID:11476466

Abstract

BACKGROUND

Lipid transfer proteins (LTPs) are highly conserved proteins present in a broad spectrum of fruits and vegetables that might represent a novel plant panallergen.

OBJECTIVE

To demonstrate that LTP is an important allergen in beer and that beer LTP cross-reacts with LTP from botanically unrelated plant-derived foods.

METHODS

Serum from a patient with clinical allergy to both beer and Rosaceae was studied for IgE reactivity to LTP to several vegetable foods by RAST, ELISA, immunoblot, and inhibition studies.

RESULTS

Patient's serum showed a strong IgE reactivity to LTP purified from peach peel, carrot, and broccoli, and to a 10 kD protein in both apple and peach immunoblots, whereas no reactivity to birch cross-reactive allergens such as Bet v 1, profilin, or carbohydrates was found. In inhibition studies, preabsorption of serum with apple, walnut, hazelnut, peanut, corn, and rice caused a fall of 97%, 20%, 66%, 91%, 94%, and 93%, respectively, of its reactivity to peach LTP. Beer RAST fell from 1.8 IU/mL to <0.1 IU/mL when a patient's serum was preabsorbed with recombinant carrot LTP.

CONCLUSIONS

LTP is a relevant allergen in beer. Beer LTP may cross-react with LTP from several other plant-derived foods.

摘要

背景

脂质转移蛋白（LTPs）是广泛存在于多种水果和蔬菜中的高度保守蛋白，可能是一种新型的植物泛过敏原。

目的

证明LTP是啤酒中的一种重要过敏原，且啤酒LTP与来自植物学上不相关的植物源性食物中的LTP发生交叉反应。

方法

通过放射性变应原吸附试验（RAST）、酶联免疫吸附测定（ELISA）、免疫印迹和抑制试验，研究了对啤酒和蔷薇科植物均有临床过敏反应的患者血清中LTP对几种蔬菜食物的IgE反应性。

结果

患者血清对从桃皮、胡萝卜和西兰花中纯化的LTP，以及苹果和桃免疫印迹中的一种10 kD蛋白显示出强烈的IgE反应性，而对桦树交叉反应性过敏原如Bet v 1、肌动蛋白 Profilin或碳水化合物无反应。在抑制试验中，用苹果、核桃、榛子、花生、玉米和大米预先吸收血清后，其对桃LTP的反应性分别下降了97%、20%、66%、91%、94%和93%。当用重组胡萝卜LTP预先吸收患者血清时，啤酒RAST从1.8 IU/mL降至<0.1 IU/mL。