猿猴腺病毒7完整和裂解DNA的生物活性
Biological activity of the intact and cleaved DNA of the simian adenovirus 7.
作者信息
Ponomareva T I, Grodnitskaya N A, Goldberg E E, Chaplygina N M, Naroditsky B S, Tikchonenko T I
出版信息
Nucleic Acids Res. 1979 Jul 11;6(9):3119-31. doi: 10.1093/nar/6.9.3119.
Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infectious titers of the DNA - TP complex for the monkey kidney cells were 30-50 times higher than those of pure DNA. Cleavage of the SA7 DNA by specific endonucleases enhanced the tumorigenic potential of pure DNA, suppressed its infectivity and did not affect the lack of transformation capacity of the DNA - TP complex. The onc-gene was localized in the left terminal fragment with the minimal size 4,3x10(6)D in the case of R.Sal I. The tumorigenic activity was found to decrease with an increase in the size of the DNA fragment containing the onc-gene.
只有7型猿猴腺病毒（SA 7）的脱蛋白DNA制剂表现出转化和致瘤活性。SA7 DNA与末端蛋白（TP）的复合物在细胞培养中既不表现出转化活性也不表现出致瘤活性。与转化潜能相反，DNA - TP复合物对猴肾细胞的感染滴度比纯DNA高30 - 50倍。用特异性核酸内切酶切割SA7 DNA增强了纯DNA的致瘤潜能，抑制了其感染性，并且不影响DNA - TP复合物缺乏转化能力的情况。在使用R.Sal I的情况下，癌基因定位在最小尺寸为4.3x10(6)D的左末端片段中。发现致瘤活性随着含有癌基因的DNA片段大小的增加而降低。
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