Altwegg M, Kubli E
Nucleic Acids Res. 1979 Sep 11;7(1):93-105. doi: 10.1093/nar/7.1.93.
The nucleotide sequence of Drosophila melanogaster phenylalanine tRNA2 was determined to be: pG-C-C-G-A-A-A-U-A-M2G-C-U-C-A-G-D-D-G-G-G-A-G-A-G-C-m22G-psi-psi-A-G-A-C(m)-U-Gm-A-A-mlG-A-psi-C-U-A-A-A-G-m7G-U(D)-C-C-C-C-G-G-T-psi-C-A-mlA-U-C-C-G-G-G-U-U-U-C-G-G-C-A-C-C-AOH. Upon RPC-5 chromatography at pH 3.8 tRNA2Phe can be separated into four isoacceptors due to the partial modifications in positions 32 and 47. Thus the posttranscriptional modification of tRNA2Phe transcribed from one gene (or many genes with identical sequences results in four isoacceptors with the same basic sequence.
黑腹果蝇苯丙氨酸tRNA2的核苷酸序列测定为:pG-C-C-G-A-A-A-U-A-M2G-C-U-C-A-G-D-D-G-G-G-A-G-A-G-C-m22G-ψ-ψ-A-G-A-C(m)-U-Gm-A-A-mlG-A-ψ-C-U-A-A-A-G-m7G-U(D)-C-C-C-C-G-G-T-ψ-C-A-mlA-U-C-C-G-G-G-U-U-U-C-G-G-C-A-C-C-AOH。在pH 3.8条件下进行RPC-5色谱分析时,由于第32位和第47位的部分修饰,tRNA2Phe可被分离为四种同功受体。因此,从一个基因(或许多具有相同序列的基因)转录而来的tRNA2Phe的转录后修饰产生了四种具有相同基本序列的同功受体。