黑腹果蝇中赖氨酸转运RNA5基因的定位
The localization of transfer RNA5Lys genes in Drosophila melanogaster.
作者信息
Grigliatti T A, White B N, Tener G M, Kaufman T C, Suzuki D T
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3527-31. doi: 10.1073/pnas.71.9.3527.
Abstract
Transfer RNA(5) (Lys) was isolated and purified from Drosophila melanogaster. It was iodinated in vitro with sodium [(125)I]iodide and hybridized in situ to the polytene salivary gland chromosomes from Drosophila. Autoradiographs exposed three to four weeks allowed the localization of this tRNA to the right arm of chromosome 2 between bands 48F and 49A.
摘要
从黑腹果蝇中分离并纯化了转运核糖核酸（5）（赖氨酸）。它在体外被[125I]碘化钠碘化，并原位杂交到果蝇的多线唾液腺染色体上。曝光三到四周的放射自显影片显示，这种转运核糖核酸定位于2号染色体右臂48F带和49A带之间。
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