Rodríguez-Ramos C, Galan F, Díaz F, Elvira J, Martín-Herrera L, Girón-González J A
Digestive Diseases, Hospital Universitario Puerta del Mar, Cádiz, Spain.
Dig Dis Sci. 2001 Aug;46(8):1668-76. doi: 10.1023/a:1010645302750.
The aim of this work was the evaluation, in cirrhotic patients with noninfected ascites and with spontaneous bacterial peritonitis (SBP), of serum and ascitic fluid levels of proinflammatory cytokines [interleukin (IL) 1-beta, tumor necrosis factor alpha (TNF-alpha), and IL6] and antiinflammatory compounds [IL10, soluble IL-1 receptor antagonist (sIL-1Ra), soluble receptors of TNF p55 and p75 (sTNFR55 and sTNFR75), and soluble receptor of IL6 (sIL6R)], as well as their relationship with the outcome of the infection in those with SBP. These molecules were assayed by ELISA in noninfected cirrhotic controls (n = 15), patients with SBP (n = 32), and healthy controls (n = 20). Serum levels of IL6 and of the majority of antiinflammatory mediators, sIL1Ra, sTNFR75, and sIL6R, were higher in control cirrhotic patients compared to healthy subjects. SBP was associated with significantly elevated ascitic fluid levels of every one of the proinflammatory cytokines compared to those in cirrhotic controls. Also, serum levels of IL10 and both TNF receptors and ascitic fluid levels of sIL1Ra and sTNFR55 were higher in patients with SBP compared to cirrhotic controls. Ascitic fluid levels of proinflammatory cytokines decreased rapidly after resolution of the infection; however, nonsignificant changes were detected in ascitic fluid concentrations of antiinflammatory molecules. Thus, elevated levels of antiinflammatory compounds both in noninfected cirrhotic patients and in patients with SBP suggest a regulatory control of the inflammatory process by these molecules in liver cirrhosis patients.
这项研究的目的是评估非感染性腹水和自发性细菌性腹膜炎（SBP）的肝硬化患者血清和腹水中促炎细胞因子[白细胞介素（IL）-1β、肿瘤坏死因子α（TNF-α）和IL-6]及抗炎化合物[IL-10、可溶性IL-1受体拮抗剂（sIL-1Ra）、TNF p55和p75的可溶性受体（sTNFR55和sTNFR75）以及IL-6的可溶性受体（sIL6R）]的水平，以及它们与SBP患者感染结局的关系。通过酶联免疫吸附测定法（ELISA）对非感染性肝硬化对照组（n = 15）、SBP患者（n = 32）和健康对照组（n = 20）检测这些分子。与健康受试者相比，肝硬化对照组患者血清IL-6及大多数抗炎介质sIL-1Ra、sTNFR75和sIL6R的水平更高。与肝硬化对照组相比，SBP患者腹水中每种促炎细胞因子的水平均显著升高。此外，SBP患者血清IL-10、两种TNF受体水平以及腹水中sIL-1Ra和sTNFR55水平均高于肝硬化对照组。感染消退后，腹水中促炎细胞因子水平迅速下降；然而，抗炎分子的腹水浓度未检测到显著变化。因此，非感染性肝硬化患者和SBP患者体内抗炎化合物水平升高表明这些分子对肝硬化患者炎症过程具有调节作用。
