Chan Y P, Chua K B, Koh C L, Lim M E, Lam S K
Institute of Biological Sciences, Faculty of Science1, Department of Medical Microbiology, Faculty of Medicine2 and Institute of Postgraduate Studies and Research3, University of Malaya, 50603 Kuala Lumpur, Malaysia.
J Gen Virol. 2001 Sep;82(Pt 9):2151-2155. doi: 10.1099/0022-1317-82-9-2151.
We have completely sequenced the genomes of two Nipah virus (NiV) isolates, one from the throat secretion and the other from the cerebrospinal fluid (CSF) of the sole surviving encephalitic patient with positive CSF virus isolation in Malaysia. The two genomes have 18246 nucleotides each and differ by only 4 nucleotides. The NiV genome is 12 nucleotides longer than the Hendra virus (HeV) genome and both genomes have identical leader and trailer sequence lengths and hexamer-phasing positions for all their genes. Both NiV and HeV are also very closely related with respect to their genomic end sequences, gene start and stop signals, P gene-editing signals and deduced amino acid sequences of nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, glycoprotein and RNA polymerase. The existing evidence demonstrates a clear need for the creation of a new genus within the subfamily Paramyxovirinae to accommodate the close similarities between NiV and HeV and their significant differences from other members of the subfamily.
我们已对两株尼帕病毒（NiV）分离株的基因组进行了全序列测定，其中一株来自马来西亚唯一一名脑脊液病毒分离呈阳性的存活脑炎患者的咽喉分泌物，另一株来自其脑脊液。这两个基因组各有18246个核苷酸，仅相差4个核苷酸。尼帕病毒基因组比亨德拉病毒（HeV）基因组长12个核苷酸，且两个基因组的前导和尾随序列长度以及所有基因的六聚体相位位置均相同。在基因组末端序列、基因起始和终止信号、P基因编辑信号以及核衣壳蛋白、磷蛋白、基质蛋白、融合蛋白、糖蛋白和RNA聚合酶的推导氨基酸序列方面，尼帕病毒和亨德拉病毒也密切相关。现有证据表明，显然需要在副粘病毒亚科内创建一个新属，以容纳尼帕病毒和亨德拉病毒之间的密切相似性以及它们与该亚科其他成员的显著差异。
