Bekkouch Oussama, Harnafi Mohamed, Touiss Ilham, Khatib Saloua, Harnafi Hicham, Alem Chakib, Amrani Souliman
Laboratory of Biochemistry and Biotechnologies, Department of Biology, Faculty of Sciences, 60050 Oujda, Morocco.
Biology Department, Sciences and Technologies Faculty, Moulay Ismail University, 52000 Errachidia, Morocco.
Evid Based Complement Alternat Med. 2019 Jul 9;2019:9734390. doi: 10.1155/2019/9734390. eCollection 2019.
Over the past decades, cardiovascular diseases have become the leading cause of death all over the world, and among these diseases there is atherosclerosis caused mainly by an increase in plasmatic cholesterol levels and by strong oxidation caused by free radicals. For these reasons and others, we explored in this report the hypolipidemic and the antioxidant effects of crude aqueous and methanolic extract. The hypolipidemic study was carried out in high-fat-fed mice model. Animals were subdivided into four groups and were orally treated with the aqueous extract once daily for twelve weeks at two doses: 250 and 500 mg/Kg. During the treatment, the body weight, total cholesterol, triglycerides, and high-density lipoproteins have been defined every four weeks. The antioxidant activity has been studied using radical scavenging activity, -carotene bleaching, reducing power assay, and the TBARs tests. The daily oral administration of the extracts for twelve weeks significantly improved the lipid profile in a dose-dependent manner, from the first until the twelfth week, and also showed a significant antioxidant effect. These findings may be potentially contributive to the validation of the medicinal use of to treat hyperlipidemia and cardiovascular complications.
在过去几十年中，心血管疾病已成为全球主要死因，在这些疾病中，动脉粥样硬化主要由血浆胆固醇水平升高和自由基引起的强烈氧化作用所致。基于这些及其他原因，我们在本报告中探讨了粗水提取物和甲醇提取物的降血脂及抗氧化作用。降血脂研究在高脂喂养小鼠模型中进行。动物被分为四组，每天口服一次水提取物，共十二周，剂量为250和500毫克/千克。治疗期间，每四周测定一次体重、总胆固醇、甘油三酯和高密度脂蛋白。使用自由基清除活性、β-胡萝卜素漂白、还原能力测定和硫代巴比妥酸反应物（TBARs）试验研究了抗氧化活性。连续十二周每日口服提取物以剂量依赖方式显著改善了血脂谱，从第一周到第十二周，还显示出显著的抗氧化作用。这些发现可能有助于验证该药物用于治疗高脂血症和心血管并发症的药用价值。
