Messinis I E, Papageorgiou I, Milingos S, Asprodini E, Kollios G, Seferiadis K
Department of Obstetrics and Gynaecology, University of Thessalia, Larissa, Greece.
Hum Reprod. 2001 Sep;16(9):1827-32. doi: 10.1093/humrep/16.9.1827.
Previous studies have alluded to a role for both oestradiol and progesterone in the secretion of leptin from fat cells in the human, although direct evidence has yet to be obtained. The study aim was to assess serum leptin concentrations in normally cycling women receiving exogenous oestradiol and progesterone.
Normally cycling women were investigated in an untreated spontaneous cycle (control, n = 10), a cycle treated with oestradiol (oestradiol cycle, n = 10) and a cycle treated with oestradiol plus progesterone (oestradiol+progesterone cycle, n = 6). Oestradiol was given to the women through skin patches on cycle days 2, 3 and 4, and progesterone intravaginally on cycle days 3, 4 and 5. Serum concentrations of leptin, oestradiol, progesterone, FSH and LH were measured in daily blood samples.
During the treatment, serum oestradiol and progesterone concentrations increased significantly. In the oestradiol cycles, leptin concentrations were not affected by treatment and did not differ from those in controls. In the oestradiol+progesterone cycles, leptin concentrations (mean +/- SEM) increased in all women from cycle day 3 (8.6 +/- 1.1 ng/ml) to days 5 (12.2 +/- 1.8 ng/ml, P < 0.01) and 6 (11.9 +/- 2.0, P < 0.05), and were at these points significantly higher than in the control cycles (P < 0.05). The mean percentage increase from day 3 to the peak concentration on days 5 or 6 was 62.6 +/- 6.8%. Leptin concentrations returned to the pretreatment value on day 7, together with the concentrations of oestradiol and progesterone. In the oestradiol+progesterone cycles, leptin concentrations correlated significantly with oestradiol and progesterone concentrations, but not with FSH and LH concentrations.
These results show, for the first time, that leptin secretion can be stimulated in women by the administration of oestradiol plus progesterone. This may explain the increased concentrations of leptin during the luteal phase of the normal menstrual cycle.
既往研究已提及雌二醇和孕酮在人体脂肪细胞分泌瘦素过程中所起的作用，尽管尚未获得直接证据。本研究旨在评估接受外源性雌二醇和孕酮的月经周期正常女性的血清瘦素浓度。
对月经周期正常的女性进行研究，包括一个未接受治疗的自然周期（对照组，n = 10）、一个接受雌二醇治疗的周期（雌二醇周期，n = 10）和一个接受雌二醇加孕酮治疗的周期（雌二醇 + 孕酮周期，n = 6）。在月经周期的第2、3和4天，通过皮肤贴片给女性施用雌二醇，并在月经周期的第3、4和5天经阴道给予孕酮。在每日采集的血样中测量瘦素、雌二醇、孕酮、促卵泡激素（FSH）和促黄体生成素（LH）的血清浓度。
在治疗期间，血清雌二醇和孕酮浓度显著升高。在雌二醇周期中，瘦素浓度不受治疗影响，与对照组无差异。在雌二醇 + 孕酮周期中，所有女性的瘦素浓度（均值±标准误）从月经周期第3天（8.6±1.1 ng/ml）升高至第5天（12.2±1.8 ng/ml，P < 0.01）和第6天（11.9±2.0，P < 0.05），且在这些时间点显著高于对照组（P < 0.05）。从第3天到第5天或第6天的峰值浓度，瘦素浓度的平均升高百分比为62.6±6.8%。在第7天，瘦素浓度连同雌二醇和孕酮浓度一起恢复到治疗前水平。在雌二醇 + 孕酮周期中，瘦素浓度与雌二醇和孕酮浓度显著相关，但与FSH和LH浓度无关。
这些结果首次表明，给予雌二醇加孕酮可刺激女性瘦素分泌。这可能解释了正常月经周期黄体期瘦素浓度升高的原因。
