Resino S, Navarro J, Bellón J M, Gurbindo D, León J A, Muñoz-Fernández M A
Department of Immunology, General University Hospital 'Gregorio Marañón', Madrid, Spain.
Clin Exp Immunol. 2001 Aug;125(2):266-73. doi: 10.1046/j.1365-2249.2001.01612.x.
The objective of this study was to investigate the relationship between peripheral blood CD4+ T cell subsets and routine viro-immunological markers in vertically HIV-1-infected children undergoing highly active antiretroviral therapy (HAART). CD4+ and CD8+ T cell subsets were examined by three-colour flow cytometry. Plasma viraemia was quantified by a standardized molecular assay. A negative correlation between the %CD4+ T cells and both viral load and the %CD8+ T cells was observed. A strong positive correlation between the %CD4 T cells and naïve, CD38+ and non-activated CD4+ T cell subsets was found, whereas the %CD4 T cells correlated negatively with the numbers of memory, activated and memory-activated CD4+ T cell subsets. Elevated percentages of CD8 T cells were associated with increased memory and CD4+ CD62L-T cell subsets, whereas the naïve and CD4+ HLA-DRCD38+ subsets negatively correlated with the CD8%. Co-expression of CD62L on memory CD4+ cells and high expression of HLA-DR (but not of CD38) were associated with high viral load. No association between viral load and naïve CD4+ T cells was observed. Specific CD4+ T cell subsets may be more informative than routine surrogate markers in defining the evolution of HIV infection and immune reconstitution in children.
本研究的目的是调查接受高效抗逆转录病毒疗法（HAART）的垂直感染HIV-1儿童外周血CD4+ T细胞亚群与常规病毒免疫标志物之间的关系。通过三色流式细胞术检测CD4+和CD8+ T细胞亚群。通过标准化分子检测法定量血浆病毒血症。观察到CD4+ T细胞百分比与病毒载量和CD8+ T细胞百分比均呈负相关。发现CD4 T细胞百分比与初始、CD38+和未激活的CD4+ T细胞亚群呈强正相关，而CD4 T细胞百分比与记忆、激活和记忆激活的CD4+ T细胞亚群数量呈负相关。CD8 T细胞百分比升高与记忆和CD4+ CD62L-T细胞亚群增加相关，而初始和CD4+ HLA-DRCD38+亚群与CD8%呈负相关。记忆CD4+细胞上CD62L的共表达和HLA-DR（而非CD38）的高表达与高病毒载量相关。未观察到病毒载量与初始CD4+ T细胞之间的关联。在确定儿童HIV感染的演变和免疫重建方面，特定的CD4+ T细胞亚群可能比常规替代标志物更具信息价值。
