Hedges J F, Demaula C D, Moore B D, McLaughlin B E, Simon S I, MacLachlan N J
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Immunology. 2001 Aug;103(4):498-504. doi: 10.1046/j.1365-2567.2001.01262.x.
Expression of E-selectin on activated endothelium is a critical initial step that leads to extravasation of leucocytes during inflammation, yet E-selectin is largely uncharacterized in several animal species including the horse. We have sequenced and compared E-selectin genes derived from activated cultures of purified equine (horse), cervid (black-tailed deer) and ovine (sheep) pulmonary artery endothelial cells (ECs). Phylogenetic and amino acid sequence comparisons indicate that bovine, cervid and ovine E-selectin are similar, whereas human and equine E-selectin are more closely related to each other than to the ruminant molecules. Human E- and P-selectin-specific monoclonal antibodies that also recognize equine E-selectin were identified and used to characterize its expression. Expression of E-selectin was more readily induced by lipopolysaccharide treatment in equine ECs than in human ECs and supported adhesion and activation of neutrophils, consistent with the extreme sensitivity of horses to endotoxaemia and septic shock.
活化内皮细胞上E-选择素的表达是炎症期间导致白细胞外渗的关键起始步骤，然而在包括马在内的几种动物物种中，E-选择素在很大程度上尚未得到充分研究。我们对来自纯化的马、鹿（黑尾鹿）和绵羊肺动脉内皮细胞（ECs）活化培养物的E-选择素基因进行了测序和比较。系统发育和氨基酸序列比较表明，牛、鹿和绵羊的E-选择素相似，而人类和马的E-选择素彼此之间的关系比与反刍动物分子的关系更为密切。已鉴定出也识别马E-选择素的人E-选择素和P-选择素特异性单克隆抗体，并用于表征其表达。与人类ECs相比，脂多糖处理更容易诱导马ECs表达E-选择素，并支持中性粒细胞的黏附和活化，这与马对内毒素血症和脓毒性休克的极端敏感性一致。
