Plante M, Jones T, Allard F, Torossian K, Gauthier J, St-Félix N, White G L, Lowell G H, Burt D S
Intellivax International Inc., 7150 Frederick Banting, Suite 200, Que., H4S 2A1, Ville St-Laurent, Canada.
Vaccine. 2001 Oct 12;20(1-2):218-25. doi: 10.1016/s0264-410x(01)00268-7.
The immunogenicity of a mucosally delivered subunit influenza vaccine was assessed in mice. Split influenza virus vaccine (sFlu) was formulated with proteosomes (Pr-sFlu), administered intranasally, and the induced immunity was compared with the responses elicited by sFlu alone given either intramuscularly or intranasally. Intranasal (i.n.) immunization with Pr-sFlu induced specific serum IgG and hemagglutination inhibition (HAI) titers comparable to or better than those induced by intramuscular (i.m.) sFlu, and in contrast to sFlu alone, i.n. Pr-sFlu also induced high levels of influenza-specific IgA in lung and nasal washes. Mice receiving i.n. Pr-sFlu were completely protected against live virus challenge, as were mice immunized by injection with sFlu alone. The i.n. Pr-sFlu elicited cytokine responses polarized towards a type 1 phenotype whereas those elicited by sFlu alone were of a mixed type 1/type 2 phenotype. The data strongly suggest that i.n. proteosome-formulated influenza antigens are highly effective and are excellent candidates for a non-invasive human vaccine.
在小鼠中评估了经黏膜递送的亚单位流感疫苗的免疫原性。裂解流感病毒疫苗（sFlu）与蛋白酶体（Pr-sFlu）混合配制，经鼻内给药，并将诱导的免疫反应与单独经肌肉注射或鼻内给予sFlu所引发的反应进行比较。用Pr-sFlu进行鼻内免疫诱导产生的特异性血清IgG和血凝抑制（HAI）效价与经肌肉注射sFlu诱导产生的效价相当或更高，并且与单独的sFlu不同，经鼻内给予Pr-sFlu还能在肺和鼻腔灌洗液中诱导产生高水平的流感特异性IgA。接受经鼻内Pr-sFlu的小鼠完全受到保护，免受活病毒攻击，单独注射sFlu免疫的小鼠也是如此。经鼻内给予Pr-sFlu引发的细胞因子反应偏向1型表型，而单独sFlu引发的反应是1/2混合型表型。数据有力地表明，经鼻内用蛋白酶体配制的流感抗原非常有效，是无创性人类疫苗的优秀候选物。
