Jain V, Das B K, Bhan M K, Glass R I, Gentsch J R
Viral Gastroenteritis Section, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 2001 Oct;39(10):3524-9. doi: 10.1128/JCM.39.10.3524-3529.2001.
We previously observed a marked diversity of rotavirus strains and a high prevalence of the uncommon serotype G9 in a small survey of rotavirus strains collected from six centers in India. In the present study, we characterized a larger collection of strains from children hospitalized with severe diarrhea in seven Indian cities between 1996 and 1998. A total of 287 strains were G and P genotyped by reverse transcription-PCR, and some were further characterized by electropherotyping and subgrouping. Of the four strains common globally, three were found in only 43% of samples (P[8], G1, 15%; P[4], G2, 22%; P[8], G4, 6%), whereas G9 strains made up 17% of the total. Three different G9 strains were present: a P[8], G9 strain, which displayed the long electropherotype and subgroup II VP6 specificity, and two P[6], G9 strains, one with the long electropherotype and subgroup II specificity and the other with the short electropherotype and subgroup I specificity. Marked diversity was observed among strains collected from different cities and collected over time. Of the 253 strains that were fully typed, 54 (21%) had a mixed G or P genotype. Serotype G2 strains were detected more often in infections caused by single strains than in mixed infections (P < 0.05), whereas serotype G1 strains were found more often in mixed infections than in infections caused by single strains (P < 0.05). The diversity of rotavirus strains and the high prevalence of mixed infections confirm trends reported earlier and help to better characterize the strains of rotavirus circulating in India. Vaccines under development should clearly target G9 strains, and G9 should be included as one of the common global serotypes.
我们之前在一项对从印度六个中心收集的轮状病毒株进行的小型调查中观察到，轮状病毒株具有显著的多样性，且罕见血清型G9的流行率很高。在本研究中，我们对1996年至1998年期间在印度七个城市因严重腹泻住院的儿童中收集的更多病毒株进行了特征分析。通过逆转录聚合酶链反应对总共287株病毒进行了G和P基因分型，一些病毒株还通过电泳分型和亚组分析进行了进一步特征分析。在全球常见的四种病毒株中，仅在43%的样本中发现了三种（P[8]，G1，15%；P[4]，G2，22%；P[8]，G4，6%），而G9病毒株占总数的17%。存在三种不同的G9病毒株：一种P[8]，G9病毒株，表现出长电泳型和亚组II VP6特异性，以及两种P[6]，G9病毒株，一种具有长电泳型和亚组II特异性，另一种具有短电泳型和亚组I特异性。在从不同城市收集以及随时间收集的病毒株中观察到显著的多样性。在253株完全分型的病毒株中，54株（21%）具有混合的G或P基因型。血清型G2病毒株在单株感染中比在混合感染中更常被检测到（P<0.05），而血清型G1病毒株在混合感染中比在单株感染中更常被发现（P<0.05）。轮状病毒株的多样性和混合感染的高流行率证实了先前报道的趋势，并有助于更好地描述在印度传播的轮状病毒株的特征。正在研发的疫苗应明确针对G9病毒株，并且G9应作为全球常见血清型之一纳入其中。
